Prolyl carboxypeptidase (PRCP) is a lysosomal proline specific serine peptidase that also plays a vital role in the regulation of physiological processes in mammals. In this report, we isolate and characterize the first PRCP in an insect. PRCP was purified from the anterior midgut of larvae of a stored product pest, Tenebrio molitor, using a three-step chromatography strategy, and it was determ...

SUMMARY In aquaculture, when alternative protein sources of Fish Meal (FM) in diets are investigated, Plant Proteins (PP) can be used. Among them, Vital Wheat Gluten (VWG) is a proteinaceous material obtained from wheat after starch extraction. "It is mainly composed of two types of proteins, gliadins and glutenins, which confer specific visco-elasticity that's to say ability to form a network ...

with tachycardia. The electrocardiogram showed diffuse repolarization abnormalities. There was a significant rise of the troponin, serum creatine kinases, and lactate dehydrogenase levels. Cardiac ultrasound confirmed the diagnosis of myocarditis. Physical examination revealed intense face paleness, mild paresis in lower limbs and abdominal distension. There was no abnormality in heart and lung...

We previously noted a region of amino acid sequence homology between A-gliadin, a major alpha-gliadin component known to activate coeliac disease, and the early region E1b protein of human adenovirus serotype 12 (Ad12), an adenovirus isolated from the human intestinal tract. In the present study sera from coeliac disease patients from the United Kingdom and the United States were assayed for ne...

The processing properties of the wheat flour are largely determined by the structures and interactions of the grain storage proteins (also called gluten proteins) which form a continuous visco-elastic network in dough. Wheat gluten proteins are classically divided into two groups, the monomeric gliadins and the polymeric glutenins, with the latter being further classified into low molecular wei...

Cereals are the basis of human nutrition; they are the food source most intensively produced in the world, surpassing 2000 million tons annually. The production of wheat, corn and rice makes up over the 70% of the total cereal production and thus dominates world agriculture. All 3 cereal crops can determine adverse reactions with different mechanisms of immune-mediated hypersensitivity and thro...

This paper presents a new hypothesis for the etiology and pathogenesis of celiac disease (CD). It is our contention that CD is triggered by the binding of one or more gliadin peptides to CD-associated HLA class II molecules. Furthermore, we propose that these putative CD peptides bind to oligosaccharide residues on HLA class II molecules distal to the peptide-binding groove invoking recognition...

WheaT gllllen proTeins have importanr functional properties. and are reliable indicators of genOType. They are also very heterogeneous. demanding use ofpoweJ:ful analytical techniques. Gel electrophoresis methods are lvidely used for gluten analysis, blll they are labor-inrensive. and their daTa are not easily quant((ied. A1ethods of capillmy electrophoresis (CE) are now available that promise ...

Gluten is the product of a chemical bond of wheat prolamin proteins (glia- dins and glutenins) in an aqueous me- dium. IgE mediated gluten allergy can be induced either by gluten as an in- gredient in foods or wheat prolamines present in the air. The aim of the study was clinical analysis of 13 patients, who demonstrated elevated levels of gluten specific IgE and identification of the most alle...