In continuation of earlier studies on murine neoplastic liver lesions, we characterized by histochemical methods the phenotype of hepatocellular adenomas and carcinomas induced by single injections of diethylnitrosamine (1.25, 2.5, or 5.0 micrograms/g of body weight) in 15-day-old C57BL/6 x male C3H F1 mice. The hepatocellular adenomas were composed predominantly of basophilic cells but stored ...

Two major species of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) differing in size, pyridine nucleotide specificity, and susceptibility to inhibition by adenosine 5'-triphosphate (ATP) were detected in extracts of Pseudomonas multivorans (which has recently been shown to be synonymous with the species Pseudomonas cepacia) ATCC 17616. The large species (molecular weight ca. 230,000) was acti...

In 1928 Miiller (1) reported the presence of a new enzyme, a glucose oxidase, in press-juice of Aspergillus niger. He subsequently suggested that it might be a dehydrogenase (2) ; he later showed that it catalyzed the oxidation of glucose to gluconic acid (3). Harrison in 1931 obtained glucose dehydrogenase from acetone-dried mammalian liver by saturating a water extract with ammonium sulfate (...

NADPH formation during growth on glucose was studied in wild type Escherichia coli and in mutants affected, directly or indirectly, in the NADPH-forming reactions glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase, and energy-linked transhydrogenase. The main technique was to grow cells in media containing glucose labeled uniformly with ‘“C and also containing glu...