By L. G. ISIIAELS, G. E. DELORY, L. HNATIUK AND E. FRIESEN T HE SERUM activity of phosphohexose isonsserase, the enszyme faeilitatinsg the coisversions of glucose-6-phosphate to fructose-6-plsosphate, has recetstly conse insto use as an inidex of tumor growth and nsetastatic disease. Bodanssky was able to demonsstrate increased serum isomerase activity ins nsetastatic carcinsonsa of breast amsd...

The deuterated water method is used extensively to measure gluconeogenesis in humans. This method assumes negligible exchange of the lower three carbons of fructose 6-phsophate via transaldolase exchange since this exchange will result in enrichment of carbon 5 of glucose in the absence of net gluconeogenesis. The present studies tested this assumption. ²H₂O and acetaminophen were ingested and ...

BACKGROUND Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion of xylose into fuels and chemicals, no study has reported the engineering of...

The following aspects of carbohydrate metabolism were compared in liver and in the transplantable hepatocarcinoma C954 carried by the C57-1eaden mouse strain: (a) glucose production from endogenous sources; (b) glucose-6-phosphatase and isomerase activities; (c) glycogen synthesis; (d) extent of glucose utilization for CO2, lactate, and fatty acid formation; (e) pathways of glucose catabolism; ...

Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals. A robust ethanol producing yeast Saccharomyces cerevisiae has been engineered with heterologous xylose assimilation pathways. A two-step oxidoreductase pathway consisting of NAD(P)H-linked xylose r...

Analysis of fructose and glucose from glucose isomerization process using immobilised glucose isomerase (IGI), {Sweetzyme from Novozymes} are often performed by HPLC methods with refractive index (RI) detector. This study is focused on developing new methods of measuring glucose and fructose using a specific carbohydrate column. The importance of this research, primarily based on the performanc...

The following aspects of carbohydrate metabolism were compared in liver and in the transplantable hepatocarcinoma C954 carried by the C57-1eaden mouse strain: (a) glucose production from endogenous sources; (b) glucose-6-phosphatase and isomerase activities; (c) glycogen synthesis; (d) extent of glucose utilization for CO2, lactate, and fatty acid formation; (e) pathways of glucose catabolism; ...

Dihydroxyacetone phosphate was prepared in 2OGmmol scale from dihydroxyacetone by two procedures: reaction with phosphorus oxytrichloride and glycerol kinase catalyzed phosphorylation using ATP with in situ regeneration of ATP by phosphoenolp5rruvate or acetyl phosphate. Dihydroxyacetone phosphate was converted to fructose 6-phosphate in 80% yield by exposure to a mixture of co-immobilized trio...

Citrus canker, caused by the bacterium Xanthomonas citri (Xcc), is one of most devastating diseases for citrus industry. Xylose a constituent cell wall plants, and ability Xcc to use this carbohydrate may play role in virulence. has two genes codifying xylose isomerase (XI), bifunctional enzyme that interconverts D-xylose into D-xylulose D-glucose D-fructose. The aim work was investigate functi...

When presented with glucose, an Escherichia coli mutant lacking both phosphoglucose isomerase and glucose 6-phosphate dehydrogenase accumulated, internally, high concentrations (approximately 50 mM) of glucose 6-phosphate. It also accumulated glucose in polysaccharide form. Other metabolism of glucose, if any, was very restricted. The accumulation of glucose 6-phosphate was associated with inhi...