Convulsions due to vitamin B-6 deficiency in humans can be controlled by the administration of pyridoxine, and the symptoms subside rapidly (Coursin, 1954). If derangement of y-aminobutyric acid metabolism were responsible for these convulsions, it would be expected that a return to normal y-aminobutyric acid metabolism occurs very rapidly after the pyridoxine administration. In vitamin B-&defi...

To characterize aspartyl aminopeptidase from Aspergillus oryzae, the recombinant enzyme was expressed in Escherichia coli. The enzyme cleaves N-terminal acidic amino acids. About 30% activity was retained in 20% NaCl. Digestion of defatted soybean by the enzyme resulted in an increase in the glutamic acid content, suggesting that the enzyme is potentially responsible for the release of glutamic...

A 6-year-old boy presented with epilepsia partialis continua 6 months after diagnosis of type 1 diabetes. Anti-glutamic acid decarboxylase 65 antibodies were found in his serum and cerebrospinal fluid. Anti-epileptic agents did not improve his seizures. High-dose steroids, plasmapheresis, and intravenous immunoglobulin resulted in decreased anti-glutamic acid decarboxylase 65 antibody levels an...

Despite extensive study of heptahelical G protein-coupled receptors (GPCRs), the precise mechanism of G protein activation is unknown. The role of one highly conserved stretch of residues, the amino acids glutamic acid/aspartic acid-arginine-tyrosine (i.e., the E/DRY motif), has received considerable attention with respect to regulating GPCR conformational states. In the consensus view, glutami...

AIMS Gestational diabetes mellitus affects approximately 7% of all pregnant women. Some of these women develop autoantibodies that are generally characteristic of Type 1 diabetes. Autoantibodies targeting glutamic acid decarboxylase and tyrosine phosphatase-like protein are the most frequently reported. A recently identified autoantigen in Type 1 diabetes is zinc transporter 8. Some reports sug...

Glutamic acid has been prepared previously by the racemization (1) of the natural dextro form and by three synthetic methods. The first synthesis, that by Wolff (2) in 1890, is principally of theoretical interest as it involves the preparation of six intermediate substances starting with levulinic acid and the yield of final product is not given. In 1925 Knoop and Oesterlin (3) obtained 1.1 gm....

Glutamic acid has been prepared previously by the racemization (1) of the natural dextro form and by three synthetic methods. The first synthesis, that by Wolff (2) in 1890, is principally of theoretical interest as it involves the preparation of six intermediate substances starting with levulinic acid and the yield of final product is not given. In 1925 Knoop and Oesterlin (3) obtained 1.1 gm....

With the advent of liquid chromatography/mass spectrometry and liquid chromatography/NMR, it has become easier to characterize metabolites that were once difficult to isolate and identify. These techniques have enabled us to uncover the existence of an alternate pathway for the disposition of glutathione adducts of several structurally diverse compounds. Studies were carried out using acetamino...