Atomistic glycoprotein simulations reveal a site-specific availability of glycan substrates in time-resolved mass spectrometry maturating enzyme kinetics.

Measurement of platelet aggregation in platelet-rich plasma (PRP) is a fundamental tool in platelet studies, despite the fact that the technique required for this is time-consuming, may need large volumes of blood, and require particular skill and special equipment. The use of a microplate reader seems useful to perform platelet aggregation more rapidly and with less material. So, the aim of th...

The multidrug resistance phenotype is one of the major problems in development of cancer cell resistance to chemotherapy. Some natural compounds from medicinal plants have demonstrated promising capacity in enhancing anticancer effects in drug resistant cancer cells. We aimed to investigate whether mangiferin might have an ability to re-sensitize MCF-7 breast cancer cells previously treated wit...

Since the recent devastating outbreak of Ebola virus disease in western Africa, there has been significant effort to understand the evolution of the deadly virus that caused the outbreak. There has been a considerable investment in sequencing Ebola virus (EBOV) isolates, and the results paint an important picture of how the virus has spread in western Africa. EBOV evolution cannot be understood...

The proteins synthesized by Friend erythroleukemic cells (line 707 B) have been studied by high-resolution polyacrylamide gel electrophoresis at various stages after the induction of erythroid differentiation by addition of dimethyl sulphoxide to the medium. After 24 h of differentiation, the rate of synthesis of most of the more abundant proteins is reduced, correlating with the reduction in t...

background and aims: herpes simplex virus type 1 (hsv1) remains a potentially serious health problem world wide. all infected people, including asymptomatic ones, are potential sources for virus transmission. virus envelope contains at least 13 glycoproteins, which glycoprotein d is the major target of immune responses. the aim of this study was development of a specific method that is a more r...

Molecularly imprinted polymers (MIPs), as inexpensive and stable substitutes of antibodies, have shown great promise in immunoassays. Glycoproteins are of significant diagnostic value. To facilitate the application of MIPs in clinical diagnostics, a general and facile imprinting method toward glycoproteins oriented for an enzyme-linked immunosorbent assay (ELISA) in the form of a 96-well microp...

antiplatelet treatment Offer a single loading dose of 300 mg aspirin as soon as possible and continue indefinitely unless contraindicated by bleeding risk or aspirin hypersensitivity. [Based on a high quality systematic review and cost effectiveness evidence] Offer a loading dose of 300 mg clopidogrel in addition to aspirin to patients with a predicted six month mortality of more than 1.5%, or ...

The aim of the present study was to establish a relationship between the results obtained with the enzyme-linked immunosorbent assay (ELISA) technique for antibodies (against bovine herpesvirus 1) in serum and those in milk at the herd level. For this purpose, 275 samples of bulk-tank milk were analysed with glycoprotein E (gE) antibody ELISA and 207 more were analysed with glycoprotein B (gB) ...