background: baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. the presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. methods: the aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

The lack of heterotrimeric G-protein homologs in the sequenced genomes of green algae has led to the hypothesis that, in plants, this signaling mechanism coevolved with the embryophytic life cycle and the acquisition of terrestrial habitat. Given the large evolutionary gap that exists between the chlorophyte green algae and most basal land plants, the bryophytes, we evaluated the presence of th...

silver nano-particles have different biological applications due to their compatibility. chemical methodsusually result in remaining some amounts of toxic reactions on the nano-particles. for this reason, the useof plants as sustainable and accessible resources in preparation of compatible nano-particles has attracteda lot of attention in the recent years. this research investigated the green s...

Marine algae have gained substantial attention from various scientific and commercial fields. The reason behind its importance is due to the rich source of bioactive compounds like proteins, carbohydrates, lipids, other pigments. algae-derived carrageenans polysaccharides are used in food, medicine, cosmetic items, gelling, emulsifying, stabilizing agents. carrageenan has also photoprotective a...

Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in inactivation of the natural fluorescence of these proteins, requiring their detection by immunolo...

There are some evidences on involvement of ABL2 in a number of cancers, especially ABL2 overexpression was observed in Raji, a Burkitt's lymphoma (BL) cell line. Therefore ABL2 overexpression may be involved in pathogenesis of Burkitt's lymphoma. The aim of this study was to evaluate ABL2 significance in BL. For this purpose 20 formaldehyde fixed paraffin embedded blocks – 16BL and 4 normal lym...

The effect of Cry proteins of Bacillus thuringiensis on the green lacewing (Chrysoperla carnea) was studied by using a holistic approach which consisted of independent, complementary experimental strategies. Tritrophic experiments were performed, in which lacewing larvae were fed Helicoverpa armigera larvae reared on Cry1Ac, Cry1Ab, or Cry2Ab toxins. In complementary experiments, a predetermine...

rhizosphere soil samples were collected from olive orchards of golestan, guilan, zanjan and qom provinces. fluorescent pseudomonad strains were isolated from the collected samples on king’s b medium and their phenotypic features characterized as based on the standard bacteriological methods. for all the tested strains oxidase reaction, fluorescent pigment, growth at 4?c and arginine dihydrolase...

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...