The ability to engineer proteins by directed evolution requires functional expression of the target polypeptide in a recombinant host suitable for construction and screening libraries of enzyme variants. Bacteria and yeast are preferred, but eukaryotic proteins often fail to express in active form in these cells. We have attempted to resolve this problem by identifying mutations in the target g...

The open reading frame alr1585 of Anabaena sp. strain PCC 7120 encodes a heme-dependent peroxidase (Anabaena peroxidase [AnaPX]) belonging to the novel DyP-type peroxidase family (EC 1.11.1.X). We cloned and heterologously expressed the active form of the enzyme in Escherichia coli. The purified enzyme was a 53-kDa tetrameric protein with a pI of 3.68, a low pH optima (pH 4.0), and an optimum r...

The electrochemical oxidation of guaiacol (2-methoxyphenol) on a treated disk of gold was studied by cyclic voltammetry. At low concentration, guaiacol undergoes one irreversible anodic peak . The current of this peak has a diffusional origin. For guaiacol concentrations higher than 4 . 10 mol dm, the voltammogram shows a post-peak. The effect of experimental data on the ratio of these two peak...

ABSTRACT. Organic-inorganic hybrid nanoflowers method with unique properties are preferred than conventional immobilization methods for the past decade. Hereemoglobin-based material (HbNFs@Cu) was synthesized under different experimental conditions (pH 5.0-9.0 and 0.01-0.50 mgmL-1 of hemoglobin) obtaining a size 9-10 µm. The encapsulation percentage weight yield HbNFs@Cu were determined as 100%...

The normal P.D. across the protoplasm of Valonia macrophysa is about 10 mv. negative (inwardly directed). On adding 0.01 M guaiacol to the sea water the P.D. becomes positive and then slowly returns approximately to the normal value. In many cases this behavior is not much affected by raising the pH and so increasing the concentration of the guaiacol ion but in other cases such an increase make...

To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxid...

The presence of guaiacol in cork stoppers is responsible for some cases of cork taint causing unpleasant alterations to wine. We have performed a characterization of the cork-associated microbiota by isolating 55 different microorganisms: eight yeast, 14 filamentous fungi or molds, 13 actinomycetes and 20 non-filamentous bacteria. A screening for degradation of vanillic acid and guaiacol produc...

Bryophytes are poikilohydric organisms that can be used as model plants to study desiccation tolerance mechanisms. The main objective of this was examine the activities antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and guaiacol (POD) in rehydrated desiccated states Syntrichia ruralis (Hedw.) Web. & Mohr. from two slopes, one North-east (NE) South-west (SW) facing collected ...

Chlorella vulqaris is a unicellular microorganism that offers health benefits due to its concentrated antioxidant production. This microalga has received huge attention natural antioxidative property as an alternative source because of rapid growth, easy and flexible culture. Research date only focuses on the growth production in selected phase, especially exponential stationary phases; however...

In thе prеsent studу, a simрle protосоl for in vitro rooting induction of the important medicinal plant Coleus forskohlii Briq with high content secondary metabolites antioxidant activity was described. For germination seeds C. were placed on MS medium suррlemented 0.4 mg L-1 gibberellic acid and 1.0 CaCl2. The оbtаinеd shoots cultured half-strength ½ containing indole-3-butyric (IBA) or indole...