This study compared the efficacy of lactoferricin B (LB), peroxyacetic acid (PAA), acidified sodium chlorite (ASC), cetylpyridinium chloride (CPC), acetic acid (AA), lactic acid (LA), acidified chlorite (AC) and water when analyzed as dipping solutions for fresh beef carcass tissue (BCT) surfaces for reducing populations of Escherichia coli O157:H7. In this study, CPC reduced E. coli O157:H7 po...

To detect Escherichia coli O157:H7 in food, a selective and differential medium, CTV-MUG-RSMAC agar, was designed. Using this medium, the growth of most interfering organisms could be inhibited, and three main biochemical characteristics, including sorbitol-negative, rahmnose-negative and MUG-negative, to identify E. coli O157:H7 could also be simultaneously recognized. Sorbitol-positive aberra...

Chlorate kills E. coli O157:H7 and has been proposed as a feed additive to be included in cattle rations immediately prior to slaughter to reduce E. coli O157: H7 populations in the gut. Antibiotic usage is not recommended in cases of E. coil O157:H7-induced hemorrhagic colitis because some antibiotics stimulate increased toxin production. This study was undertaken to determine if chlorate trea...

A novel surface plasmon resonance (SPR) biosensor using lectin as bioreceptor was developed for the rapid detection of Escherichia coli (E. coli) O157:H7. The selective interaction of lectins with carbohydrate components from bacterial cells surface was used as the recognition principle for the detection. Five types of lectins from Triticum vulgaris, Canavailia ensiformis, Ulex europaeus, Arach...

The protein kinase inhibitor H7 blocks influenza virus replication, inhibits production of the matrix protein (M1), and leads to a retention of the viral ribonucleoproteins (vRNPs) in the nucleus at late times of infection (K. Martin and A. Helenius, Cell 67:117-130, 1991). We show here that production of assembled vRNPs occurs normally in H7-treated cells, and we have used H7 as a biochemical ...

Escherichia coli (E. coli) O157:H7 is a Shiga toxin producing member of enterohemorrhagic E. coli (STEC). It is a major foodborne bacterial pathogen, which was first associated with human illness during an outbreak of hemorrhagic colitis in 1982, and has been listed by the Centers for Disease Control and Prevention as a national notifiable disease since 1994. This bacterium has been estimated t...

Leafy green produce has been associated with numerous outbreaks of foodborne illness caused by strains of Escherichia coli O157:H7. While the amounts of culturable E. coli O157:H7 rapidly decline after introduction onto lettuce in the field, it remains to be determined whether the reduction in cell numbers is due to losses in cell viability, cell injury and a subsequent inability to be detected...

Feedlot cattle slaughtered at a large abattoir in northern Italy during 2002 were examined for intestinal carriage and carcass contamination with Escherichia coli O157:H7. Carcass samples were taken following the excision method described in the Decision 471/2001/EC, and fecal material was taken from the colon of the calves after evisceration. Bacteria were isolated and identified according to ...