The structure of the Gram-positive flagellum is poorly understood, and Bacillus subtilis encodes three proteins homologous to the flagellar hook protein from Salmonella enterica. Here we generated a modified B. subtilis hook protein that could be fluorescently stained using a cysteine-reactive dye. We used the fluorescently labeled hook to demonstrate that FlgE is the hook structural protein an...

Conditions influencing the opening of the bean (Phaseolus vulgaris L.) and cotton (Gossypium hirsutum L.) hypocotyl hook were defined. Such hooks were shown to undergo geotropic curvature; orientation of the hook with respect to gravity greatly affected the observed opening. Cotton and bean hooks behaved exactly opposite in regard to the presence of the cotyledons and apical bud. The cotton hoo...

INTRODUCTION The objective of this study was to demonstrate the potential of 3-dimensional modeling and finite element analysis as clinical tools in treatment planning for orthodontic tooth movement. High stresses in bone and miniscrew implants under load can cause fractures and trauma for orthodontic patients, and treatments are typically planned by using clinical experience or simple 2-dimens...

To cite: di Summa PG, Yvon A, Raffoul W, et al. BMJ Case Rep Published online: [please include Day Month Year] doi:10.1136/ bcr-2014-204779 DESCRIPTION A 34-year-old man, right-handed, smoker, presented to A&E with a 2-month history of pain, numbness and cold sensitivity in the fourth and fifth digits of his right hand. He had not suffered from any recent trauma; his usual activity was carpentr...

During flagellar morphogenesis in Salmonella typhimurium, the flagellum-specific anti-sigma factor FlgM is exported out of the cells only after completion of hook assembly. In this study, we examined the export of the flagellar proteins, FlgD (hook capping protein), FlgE (hook protein), FlgK and FlgL (hook-filament junction proteins), FliD (filament capping protein), and FliC (flagellin), befor...

The kinematics of an arresting-hook unit are studied in order to determine, within the limits of the assumption of a perfectly rigid hook unit, the damper force necessary to control hook bounce. The necessity for a smooth deck and the desirability of small trail angle for the hook unit are demonstrated from several aspects. The design requirements for a hook damper unit are discussed in all the...

Subsurface hook formation during initial solidification in the continuous casting mould degrades the quality of steel slabs owing to the associated entrapment of argon bubbles and non-metallic inclusions. To minimise hook depth and to improve slab quality, extensive plant experiments were performed and analysed to quantify the effect of casting parameters on hook characteristics using the no. 2...