Triticum aestivum is of major importance both nutritionally and economically globally. Traditional breeding mechanisms have been unsuccessful at keeping pace with the increasing demand for better yielding and more resilient wheat varieties. The introduction of foreign genes into systems has provided a new tool for crop improvement, but has been difficult to apply to elite wheat varieties mainly...

Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR/Cas9 introduced as DNA or RNA. This transient expression-based genome-editing system is highly e...

Hard red winter wheat was treated with pirimiphos-methyl at 4, 6 and 8 mg kg(-1), synergized pyrethrins at 0.38, 0.75, 1.13 and 1.5 mg kg(-1), and combinations of the two insecticides, to conduct laboratory bioassays against four beetle pests of stored grain, red flour beetle Tribolium castaneum (Herbst), rusty grain beetle Cryptolestes ferrugineus (Stephens), lesser grain borer Rhyzopertha dom...

Cereal Chem. 83(5):529–536 The accuracy of using near-infrared spectroscopy (NIRS) for predicting 186 grain, milling, flour, dough, and breadmaking quality parameters of 100 hard red winter (HRW) and 98 hard red spring (HRS) wheat and flour samples was evaluated. NIRS shows the potential for predicting protein content, moisture content, and flour color b* values with accuracies suitable for pro...

Analyses for test weight, 500-kernel weight, protein content, ash content, and hardness characteristics of fractions ot 13 samples of hard red winter wheat, obtained b>spectrometric separation, revealeci high negatile correlations in most cases between plumpness factors and protein and ash contents, and high positive correlations between plumpness factors and hardness, when the latter is determ...

The digestibility of the starch component of raw cereals in newly weaned piglets is highly variable. Reasons for this must be elucidated if the most suitable cereals are to be used. A novel approach was employed, which consisted of assessing the physicochemical properties (rapid visco analysis, water absorption and solubility indices, particle size distribution and in vitro amylolytic digestion...