IntroductionFHR1 is a member of Factor H (FH) protein family involved in complement to regulate the innate immune response. FHR1 shares with FH highly conserved C-terminal region and considered act as competitor cause induction. also an inflammatory mediator able inhibit terminal activation. Homozygous deletion FHR1, found occur 10% healthy population, strongly associated pathologies like atypi...

Using thin-layer agarose gel isoelectric focusing overlaid with thin-layer erythrocyte agar plates, we found that crude streptolysin O (SLO) consisted of a variety of hemolytic components with different isoelectric points (pIs) and that the distribution of pIs in crude SLO was different even in samples which were produced from a single strain of Streptococcus pyogenes under similar conditions. ...

An automated fluorescence polarization immunoassay for the determination of vancomycin levels in serum was evaluated. The vancomycin assay is a homogeneous competitive inhibition immunoassay based on changes in fluorescence polarization that occur with antibody binding. This assay was compared with a liquid chromatographic assay and an agar well diffusion bioassay method by using clinical serum...

A protein A-hemolytic plaque assay was applied to detect immunoglobulin (Ig)-producing cells in horse peripheral blood, using pokeweed mitogen as a B lymphocyte activator. A maximum number of Ig-secreting cells was obtained when horse peripheral blood lymphocytes were cultured in a medium containing horse serum. The number of Ig-secreting cells in young horses (2 years old) was lower than that ...

Monomer 2-hydroxy ethyl methacrylate containing small amounts of poly(caprolactone) and poly(ethylene glycol) incorporated with an antibiotic ofloxacin was polymerized by photopolymerization technique using 2,4,6 trimethyl benzoyl diphenyl phosphine oxide (TPO) as photoinitiator. Encapsulation efficiency and in vitro drug release was studied using UV-visible spectroscopy. Swelling analysis was ...

Numerous therapeutic peptides do not enter the clinical trials just because of their high hemolytic activity. Recently, we developed a database, Hemolytik, for maintaining experimentally validated hemolytic and non-hemolytic peptides. The present study describes a web server and mobile app developed for predicting, and screening of peptides having hemolytic potency. Firstly, we generated a data...

T HE ERYTHROCYTES of patients with Paroxysmal Nocturnal Hemoglobinuria (PNH) hemolyse at 37 C. in normal compatible human sera in the absence of demonstrable antibody. The reaction is complement dependent and in vivo causes a hemolytic anemia of varying severity. The diagnostic acid hemolysis test for this disorder is performed by demonstrating hemolysis of suspect erythrocytes in the patient’s...