Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

background and aims: the latency-associated transcript (lat) transcribed by latent herpes simplex virus type-1 in neuron cells are able to influence their host cell pathways. while the most of previous studies were focused on anti-apoptotic effects of lat, our investigation is making an effort to explore lat potency on cell cycle pathway in neuroblastoma cell lines. methods: the evaluation of l...

a complication in the analysis of the role of icp34.5 gene in the herpes simplex virus type-1 (hsv-1) lifecycle is the presence of overlapping antisense gene, open reading frame p (orf p), which is also deleted in hsv-1 icp34.5 negative mutants. a hsv-1 wild type strain (17+) icp34.5/orf p deletion mutant (1716) is totally avirulent in animal models and impaired in a number of in vitro function...

A protocol for testing cerebrospinal fluid specimens using a range of PCR assays for the diagnosis of central nervous system infection was developed and used to test prospectively 383 specimens. PCR assays were used for the detection of adenovirus, Borrelia burgdorferi, enteroviruses, Epstein Barr virus, cytomegalovirus, herpes simplex virus, human herpes virus type 6, JC virus, Leptospira inte...

Although varicella zoster virus latency has been demonstrated in several sensory ganglia, herpes zoster usually effects only one single, either left or right, dermatome in half of the body. In immunocompromised patients, more than one contiguous unilateral dermatome may be involved. Bilateral non-contiguous herpes zoster, also termed herpes zoster duplex, is rarely reported. Chronic varicella z...

BACKGROUND Human pathogens that can cause infertility may also affect sperm count and quality. Viral infections can be considered as direct and/or indirect cause of male factor infertility. OBJECTIVE Our goal was to investigate the prevalence of herpes simplex virus in the semen of infertile men attending the Avicenna Infertility Clinic, and to compare it with the herpes virus serology result...

What is genital herpes? Genital herpes (HER-pees) is a disease caused by a virus called herpes simplex. It may cause painful blisters on the thighs, buttocks, or genitals. The blisters break open and turn into sores. The virus can also cause fever blisters or cold sores around the mouth. Once you get herpes, the virus stays in your body for the rest of your life. When sores form, it is called a...

The varicella zoster virus primarily causes chickenpox. Herpes zoster is a typical late manifestation with reactivation of latent virus in sensory ganglia. Chickenpox is a highly contagious disease. Herpes zoster has been assumed to be less contagious, but studies have shown that reactivation of the varicella zoster virus also leads to significant spread of the virus (1–4). There has been incre...

5-Bromodeoxycytidine is phosphorylated to 5-bromodeoxycytidine 5'-monophosphate in extracts of cells infected with herpes simplex virus but not in extracts of uninfected cells. The conversion of 5-bromodeoxycytidine to nucleotides and its utilization for DNA synthesis in uninfected cells occurs by deamination of 5-bromodeoxycytidine to 5-bromodeoxyuridine followed by phosphorylation of 5-bromod...

An enzyme-linked immunosorbent assay (ELISA) kit for herpes simplex virus developed by Ortho Diagnostic Systems, Inc., was evaluated. In phase I experiments, 263 clinical specimens from genital lesions were extracted into serum-free medium and then tested by ELISA for herpes simplex virus antigen. The results were compared with those obtained by conventional viral culture. Of 83 specimens, 65 w...