Systemic delivery of specific therapeutic proteins by a parenteral route of administration is a recognized practice in the management of several gene defects and acquired diseases. As an alternative to repetitive parenteral administration, gene therapy may provide a novel means for systemic delivery of therapeutic proteins while improving patient compliance and therapeutic efficacy. However, fo...

A recombinant gene encoding human growth hormone (hGH) was stably introduced into cultured myoblasts with a retroviral vector. After injection of genetically engineered myoblasts into mouse muscle, hGH could be detected in serum for 3 months. The fate of injected myoblasts was assessed by coinfecting the cells with two retroviral vectors, one encoding hGH and the other encoding beta-galactosida...

Radioimmunoassay of human growth hormone (HGH) using adsorption to talc was examined. Results are as follows: 1) The minimal detectable amount of HGH was 0.02 ng/ml. The mean recovery rate was 100.8%. Mean +standard error (S.E.) of the same two samples, repeatedly measured eight times, were 3.5+0.2 and 35.4+0.5 ng/ml, respectively . Concentrations of the same acromegalic plasma diluted in diffe...

Serum levels of prolactin (PRL) and Human Growth Hormone (HGH) were assayed in 38 male alcoholics and 24 male control subjects using radioimmunoassay (RIA) technique. Biochemical parameters of hepatic function and severity of withdrawal state were also assessed. Significantly elevated values of plasma HGH were found in alcoholics as a group. Nineteen percent and eight percent of the patient had...

we studied the expression of human growth hormone (hgh) in e. coli under a bacteriophage t5-base promoter in a pqe30 expression vector. for an efficient expression of hgh cdna, a number of codons at the hgh n-terminal coding region were altered based on the e. coli major codons. an over-expression of hgh in the bacteria, carrying the recombinant plasmids, was observed at 37°c in the presence of...

Deamidation of proteins is probably one of the most common chemical degradation pathways in protein pharmaceuticals. The reaction rate for deamidation of human growth hormone (hGH) is particularly pH-dependent and occurs typically faster at neutral and alkaline pH [1]. Protamine from salmon is an extremely basic peptide due to its high arginine content. It is isolated from sperm cells of salmon...

It has been generally recognized that human growth hormone (HGH) has the effect to facilitate growth in cases of pituitary dwarfism. As of April 1970, however, there are only 33 reported cases in the world in which HGH has been administered for more than two years (Soyka et al., 1970). We have treated 14 cases of pituitary dwarfism with HGH, and observed initial increases in growth rate in all ...

A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in th...

Previous studies showed hyperre-sponsiveness to human growth hormone (hGH) in men with myotonic or limb girdle dystrophies (MMD or LGD). Because polyamines may mediate some actions of hGH, we have now investigated polyamine metabolism in these and other dystrophies. Under metabolic balance study conditions, serum and urine levels of putrescine (Pu), spermidine (Sd), spermine (Sm), and cadaverin...