The present study was carried out to examine the capability of saturated sodium chloride solution as a fixative agent instead of formalin which is regarded as a carcinogenic material. For this purpose 3 rabbits were used and their livers, kidneys and spleens were exposed and removed. Neutral buffered formalin solution, saturated sodium chloride solution and distilled water were used as fixative...

Background: Microwave technology has revolutionized histological processing by reducing processing time and improving tissue integrity. We describe the first microwave-accelerated tissue processing procedure for healthy and diseased tissue fragments of two species of corals: the gorgonian Gorgonia ventalina and the scleractinian Acropora cervicornis. Methods: Fourteen tissue samples from sea fa...

Computerized tomography assisted Stereotactic biopsy technique using Leksell stereotactic frame was performed on 27 patients with small, multiple and deep seated brain tumours. There were 19 men and 8 women with an age range from 17 to 65 years. Histological diagnosis of 18 glial tumours, 9 non-glial tumours (5 colloid cysts, 4 metastatic lesions) was obtained. There was no mortality and minima...

Thirty-five patients with deep brain tumours have been submitted to transtumoral stereotactic impedance monitoring and serial biopsy. The direct examination of the biopsy samples confirmed the presumptive clinical and neuroradiological diagnosis in 25 patients, but in 10 patients the histological diagnosis differed from the presumptive one. In this second group the treatment was changed as a re...

BACKGROUND To investigate pulp response to the application of two types adhesive systems (total-etch and self-etch) in human premolar teeth. MATERIALS AND METHODS Cavities limited to enamel walls in all margins with 2.5 mm depth were prepared on buccal surfaces of thirty three human premolars. The cavities were treated with the following adhesive. Single Bond (SB) and Prompt L-Pop (PLP). The ...

This study describes aspects of the infection caused by the myxosporean genus Henneguya, which forms cysts in the bony portion of the gill filaments of Hypophthalmusmarginatus. Specimens of this catfish were acquired dead from artisanal fishermen near the town of Cametá, state of Pará, northern Brazil, between July 2011 and May 2012. They were transported in refrigerated containers to the Carlo...

BACKGROUND DNA cytometry is a technique that allows densitometric analysis of nuclear DNA of cells and in this way the evaluation of DNA ploidy. OBJECTIVE The purpose of the present study was the evaluation of DNA ploidy in oral leukoplakia and oral lichen planus and correlation with histological dysplasia. METHODS We analyzed oral incisional biopsies from a group of 40 patients with oral l...

The freeze substitution histological technique allows the indirect light microscopicobservation and the quantitative evaluation of ice crystal size in frozen tissues. Theuseofchemical fixatives in substitu ting fluids improves morphological and histochemical preservation of the tissue. Fixation conditions become important since this step can introduce modifications in crystal sizes . Effects of...

The histological and histomorphometrical examination were the gold standard in the qualitative and quantitative analyses of the peri-implant tissue around the implant. In recent years, the field of microscopy has witnessed a considerable enhancement of the performance of microscopes that have very high resolution performance and allowing very sophisticated analysis even larger than traditional ...