Penyakit tuberkulosis merupakan suatu gangguan pernapasan yang menular dan penyebabnya dari adanya infeksi bakteri Mycobacterium Tuberkulosis (bakteri aerob). Pengobatan tuberculosis biasa digunakan obat lini pertama yakni isoniazid, rifampisin, etambutol pirazinamid. Tujuan penelitian ini adalah untuk meninjau efektivitas pengembangan metode HPLC atau UPLC dengan detektor UV MS konsentrasi dal...

An HPLC-UV/MS method has been developed to identify and quantify flame retardants in post-consumer plastics from waste of electric and electronic equipment (WEEE). Atmospheric pressure chemical ionisation spectra of 15 brominated and phosphate-based flame retardants were recorded and interpreted. The method was applied to detect flame retardant additives in polymer extracts obtained from pressu...

Nanoemulsions (NEs) and Lipid Nanoparticles(LNs) are regarded as suitable nanosized carriers of water-insoluble active components such as Alpha Lipoic Acid (ALA), Coenzyme Q10 (CoQ10) and Vitamin E (VE) to improve the physical and chemical stabilities of active components. Good physical and chemical stability of particles was proved by Photon Correlation Spectroscopy (PCS), Laser Diffraction (L...

A high-performance liquid chromatography (HPLC) method for the determination of azadirachtin (A and B) residues in bovine muscle has been developed. Azadirachtin is a neutral triterpene and chemotherapeutic agent effective in controlling some pest flies in horses, stables, horns and fruit. The actual HPLC method uses an isocratic elution and UV detection. Liquid-liquid extraction and solid-phas...

An HPLC/UV method was first optimized for the separation and quantitation of human acylated and unacylated (or des-acyl) ghrelin from aqueous solutions. This method was validated by an original approach using accuracy profiles based on tolerance intervals for the total error measurement. The concentration range that achieved adequate accuracy extended from 1.85 to 59.30microM and 1.93 to 61.60m...

Monomeric and oligomeric proanthocyanidins present in a range of plant-derived foods and beverages were separated by degree of polymerization and identified using a modified normal-phase high-performance liquid chromatography (HPLC) method coupled with on-line mass spectrometry (MS) analysis using an atmospheric pressure ionization electrospray chamber. In addition, ultraviolet (UV) and fluores...

In the last 15 years NMR has become a reference technique for the determination of purity for organic standards containing NMR active nuclei [1, 2]. The puzzle proposed for this Analytical Challenge was focused on the use of 1H-NMR for the characterization of a phenylbutazone standard. Such material was purchased from Sigma-Aldrich and analyzed in parallel by HPLC-UV and 1H-NMR. Initially, appl...

The dried aerial parts of the mountain tea Sideritis euboea were extracted using n-hexane, methanol, diethyl ether, ethyl acetate, and n-butanol. The residues were tested for their antioxidant activity on sunflower oil at 50 degrees C under UV light. The oxidation of the sunflower oil was measured using PV, absorbance E(1%)1 cm, and malondialdehyde by high-performance liquid chromatography (HPL...

An aqueous normal phase HPLC method coupled with UV or ESI/MS detection was used for the determination of a wide variety of nucleotides, essential in metabolomics studies. Fifteen nucleotides were tested in clinically relevant mixtures at levels of 100 microg/mL for UV detection and 1 microg/mL for ESI-MS detection. Analysis times for all protocols developed were less than 20 min. The chromatog...

An HPLC-UV analytical method was developed to determine ethylenediaminetetraacetic acid (EDTA) in dairy wastewater and surface water. The optimizing separation was achieved by reversed–phase ion-pair liquid chromatography on a C18 column using methanol as mobile phase solvent, tetrabutylammonium bromide as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate of 0.9 mL min with ...