نتایج جستجو برای: hrm respectively

تعداد نتایج: 688499  

Journal: :Journal of clinical microbiology 2012
Wei Li Masanori Matsuoka Masanori Kai Pratibha Thapa Saraswoti Khadge Deanna A Hagge Patrick J Brennan Varalakshmi Vissa

Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to investigate ongoing transmission of leprosy in regions of endemicity. To enable wider implementation of these molecular analyses, novel real-time PCR-high-resolution melt (RT-PCR-HRM) assays without allele-specific primers or probes and post-PCR sample handling were developed. For the detection of mutations ...

2014
Min Lin Ji-Wei Jiao Xiu-Hui Zhan Xiao-Fen Zhan Mei-Chen Pan Jun-Li Wang Chun-Fang Wang Tian-Yu Zhong Qin Zhang Xia Yu Jiao-Ren Wu Hui-Tian Yang Fen Lin Xin Tong Hui Yang Guang-Cai Zha Qian Wang Lei Zheng Ying-Fang Wen Li-Ye Yang

β-thalassemia is a common inherited disorder worldwide including southern China, and at least 45 distinct β-thalassemia mutations have been identified in China. High-resolution melting (HRM) assay was recently introduced as a rapid, inexpensive and effective method for genotyping. However, there was no systemic study on the diagnostic capability of HRM to identify β-thalassemia. Here, we used a...

2007
Tomasz K. Wojdacz Alexander Dobrovic

In this article, we show that high resolution melting analysis (HRM) is a sensitive and specific method for the detection of methylation. Methylated DNA and unmethylated DNA acquire different sequences after bisulphite treatment resulting in PCR products with markedly different melting profiles. We used PCR to amplify both methylated and unmethylated sequences and assessed HRM for the determina...

Journal: :Journal of clinical microbiology 2011
Hanna Woksepp Cecilia Jernberg Maria Tärnberg Anna Ryberg Alma Brolund Michaela Nordvall Barbro Olsson-Liljequist Karin Tegmark Wisell Hans-Jürg Monstein Lennart E Nilsson Thomas Schön

Methods for the confirmation of nosocomial outbreaks of bacterial pathogens are complex, expensive, and time-consuming. Recently, a method based on ligation-mediated PCR (LM/PCR) using a low denaturation temperature which produces specific melting-profile patterns of DNA products has been described. Our objective was to further develop this method for real-time PCR and high-resolution melting a...

2012
Alexander Dobrovic

In this article, we show that high resolution melting analysis (HRM) is a sensitive and specific method for the detection of methylation. Methylated DNA and unmethylated DNA acquire different sequences after bisulphite treatment resulting in PCR products with markedly different melting profiles. We used PCR to amplify both methylated and unmethylated sequences and assessed HRM for the determina...

2005
Alex Bryson Rafael Gomez Tobias Kretschmer

In this paper we treat workplace voice and systems of high-commitment human resource management (HCHRM) as technological innovations in order to account for the uneven diffusion patterns observed across establishments. Using British data, the paper finds that variables highlighted in the technological diffusion literature are significant predictors of voice and HRM adoption decisions. Workplace...

2010
Kamphol Wipawayangkool

This paper overall aims to encourage researchers and managers to consider the role of human resource management (HRM) in the field of information security management (ISM) more seriously. This paper suggests that with more strategically active role of HRM through a combination of selection, training, and pay practices, organizations not only can manage people issues in ISM particularly security...

Journal: :Ethnicity & disease 2007
Ruth Enid Zambrana Carma Ayala Olivia Carter Pokras Jasmin Minaya George A Mensah

OBJECTIVES To compare hypertension-related mortality (HRM) age-standardized and age-specific rates for Hispanic subgroup and non-Hispanic White (NHW) women; to identify underlying causes of HRM by Hispanic subgroup and age; and to examine relative percent change in HRM among Hispanic subgroups and NHW women. DESIGN Secondary data analyses of 1995-1996 and 2001-2002 national vital statistics m...

2012
Patiyan Andersson Steven Y. C. Tong Jan M. Bell John D. Turnidge Philip M. Giffard

Here we report a single nucleotide polymorphism (SNP) based genotyping method for Klebsiella pneumoniae utilising high-resolution melting (HRM) analysis of fragments within the multilocus sequence typing (MLST) loci. The approach is termed mini-MLST or Minim typing and it has previously been applied to Streptococcus pyogenes, Staphylococcus aureus and Enterococcus faecium. Six SNPs were derived...

Journal: :Health Research Policy and Systems 2009
Marjolein Dieleman Barend Gerretsen Gert Jan van der Wilt

BACKGROUND Improving health workers' performance is vital for achieving the Millennium Development Goals. In the literature on human resource management (HRM) interventions to improve health workers' performance in Low and Middle Income Countries (LMIC), hardly any attention has been paid to the question how HRM interventions might bring about outcomes and in which contexts. Such information is...

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