BACKGROUND Molecular tests allow the detection of high-risk human papillomavirus in cervical samples, playing an important role in the prevention of cervical cancer. OBJECTIVES We performed a study to determine the prevalence of HPV 16, HPV 18 and other high-risk human papillomavirus (pool 12 genotypes) in Peruvian females from diverse urban areas using the cobas 4800 HPV test. METHODS Rout...

Oral human papillomavirus type16 (HPV16), a sexually transmitted infection, is believed to be responsible for the rise in incidence of oropharyngeal squamous cell cancers in the United States.1 An association between self-reported number of cigarettes currently smoked per day and oral HPV prevalence has been observed.2,3 We investigated associations between objective biomarkers reflective of al...

Inactivation of the ARF-p53 tumor suppressor pathway leads to immortalization of murine fibroblasts. The role of this pathway in immortalization of human epithelial cells is not clear. We analyzed the functionality of the p14(ARF)-p53 pathway in human mammary epithelial cells (MEC) immortalized by human papillomavirus 16 (HPV16) E6, the p53 degradation-defective E6 mutant Y54D, or hTERT. E6-MEC...

OBJECTIVES The aim of this study was to identify highly oncogenic forms of human papillomavirus in the oral mucosa of asymptomatic men. METHODS In this study, we analyzed samples of exfoliated cells from the oral cavity of 559 asymptomatic men. DNA-human papillomavirus was detected using the consensus primers PGMY09/11; viral genotyping was performed using type-specific PCR and restriction fr...

background: the most common laryngeal mass in children is recurrent respiratory papillomatosis (rrp). studies have attempted to correlate viral typing and its aggressiveness. method: 29 patients with histologically confirmed rrp enrolled in adjuvant therapies. patients underwent several surgical interventions. results: hpv genotyping demonstrated 45% hpv-6 and 55% hpv-11. the mean age at the fi...

Human papillomavirus type 16 (HPV-16) is a major cause of human cancer. Effective prophylactic vaccines are based on type-specific neutralizing antibodies. A major neutralizing epitope has been defined by the monoclonal antibody H16.V5. To investigate the importance of this epitope for overall immunogenicity of HPV-16, HPV-16 virus-like particles devoid of the H16.V5 epitope were engineered by ...

BACKGROUND Human papillomavirus vaccines prevent human papillomavirus infection and cervical precancers. The impact of vaccinating women with a current infection or after treatment for an human papillomavirus-associated lesion is not fully understood. OBJECTIVES To determine whether human papillomavirus-16/18 vaccination influences the outcome of infections present at vaccination and the rate...