patients and methods this prospective cohort study was performed on 90 diabetic type 2 patients with microalbuminuria, who were selected according to the consecutive sample selection method during 6 years. data were collected through regular and systematic measurements of serum albumin as the response variable and body mass index, systolic and diastolic blood pressure, the duration of diabetes,...

Abstract Interactions between pentacyclic triterpenes isolated from white birch (Betula pendula Roth.) bark samples Northeast Bulgaria and bovine serum albumin or human were investigated using fluorescence techniques. The experimental results show the formation of complexes with albumins. Quenching intrinsic albumins was monitored by emission spectra varied quencher concentration solutions. By ...

Human liver sections were stained with anti-human serum albumin and/or anti-human fibrin monomer fluorescent conjugates. Approximately 10 per cent of the hepatic cells stained specifically for human serum albumin,1 per cent for fibrinogen, and 0.1 per cent for both. Approximately 18 per cent of the Kupffer cells stained specifically for human serum albumin and 33 per cent for fibrinogen. Staini...

In previous work the existence of free thyroxine in human serum was verified by dialysis through cellophane; the concentration of the unbound hormone in normal human serum was found to approximate one-thousandth of the total thyroxine (1). This direct measurement of free thyroxine was in reasonable agreement with the theoretical value computed from data on the per cent of total thyroxine bound ...

In this solid-phase competitive enzymoimmunoassay for albumin in human urine, antiserum to human serum albumin labeled with horseradish peroxidase (EC 1.11.1.7) is incubated with solid-phase-bound human serum albumin in the presence of sample or standard. Results obtained correlate well (r = 0.96) with those of an established fluoroimmunoassay. The present assay covers the range 0.9 to 200 mg/L...

The interaction of the immobilized triazine dye Cibacron Blue 3G-A with rat, rabbit, sheep, goat, bovine and human serum albumins was studied by affinity gel electrophoresis. Dissociation constants were estimated in each instance and showed human serum albumin to have a significantly higher affinity for the dye than did albumin from any other species. Pretreatment of the defatted proteins with ...

BACKGROUND Albumin is the most abundant protein in blood plasma, and due to its ligand binding properties, serves as a circulating depot for endogenous and exogenous (e.g. drugs) compounds. Hence, the unbound drug is the pharmacologically active drug. Commercial human albumin preparations are frequently used during surgery and in critically ill patients. Recent studies have indicated that the u...

2.1.Date of QMRF: 06.10.2009 2.2.QMRF author(s) and contact details: [1]Indrek Tulp Molcode Ltd. Turu 2, Tartu, 51014, Estonia [email protected] http://www.molcode.com [2]Tarmo Tamm Molcode Ltd. Turu 2, Tartu, 51014, Estonia [email protected] http://www.molcode.com [3]Gunnar Karelson Molcode Ltd. Turu 2, Tartu, 51014, Estonia [email protected] http://www.molcode.com [4]Dimitar Dobchev Molcod...

The features of midazolam (MDZ) induced structural perturbation of human serum albumin (HSA) has been studied in detail by Fourier transformation infrared spectra (FT-IR),and circular dichroism (CD).The CD spectra was deconvoluted to quantify the changes in α helix, β sheets, β turns and random coils of the protein. UV-Vis absorption studies suggested the static type quenching procedure for thi...