Actinopyga lecanora, a type of sea cucumber commonly known as stone fish with relatively high protein content, was explored as raw material for bioactive peptides production. Six proteolytic enzymes, namely alcalase, papain, pepsin, trypsin, bromelain and flavourzyme were used to hydrolyze A. lecanora at different times and their respective degrees of hydrolysis (DH) were calculated. Subsequent...

To determine the angiotensin-converting enzyme (ACE) inhibitory activity of a fish hydrolysate, different methods were tested. Finally, a sensitive, extraction-free HPLC method using N-(3-[2-furylacryloyl)-PheGly-Gly (FAPGG) as substrate was preferred. This method relies on the UV-titration of the peptide 2-furylacryloyl-L-Phe (FAP) resulting from the hydrolysis of the FAPGG after a chromatogra...

OBJECTIVES To review the current status of collagen hydrolysate in the treatment of osteoarthritis and osteoporosis. METHODS Review of past and current literature relative to collagen hydrolysate metabolism, and assessment of clinical investigations of therapeutic trials in osteoarthritis and osteoporosis. RESULTS Hydrolyzed gelatin products have long been used in pharmaceuticals and foods;...

Lyophilization stabilizes the biological materials by using two overlapping drying procedure i.e. primary drying by sublimation of the ice crystal from frozen material and secondary drying or desorption by evaporation of the free water adsorbed into the dried product. Three different stabilizers i.e. lactalbumin hydrolysate-sucrose, Weybridge medium and lactalbumin hydrolysate-manitol were used...

An experiment to determine the chemical composition and protein quality of 13 fish substrates (pollock by-products, n = 5; fish protein hydrolysates, n = 5; and fish meals, n = 3) was conducted. Two of these substrates, salmon protein hydrolysate (SPH) and salmon meal with crushed bones (SMB), were used to determine their palatability as components of dog diets. Pollock by-products differed in ...

WESSMAN, G. E. (National Animal Disease Laboratory, Ames, Iowa). Cultivation of Pasteurella haemolytica in a casein hydrolysate medium. Appl. Microbiol. 13:426-431. 1965.-The growth of Pasteurella haemolytica strain H44L was studied under aerobic conditions in a medium of acid-hydrolyzed casein, supplementary cysteine, inorganic salts, vitamins, and a carbon source. The concentration of casein ...

Phenolic compounds are important inhibitors of the microorganisms used in the Acetone-Butanol-Ethanol (ABE) fermentation. The degradation of phenolic compounds in a wood pre-hydrolysate, a potential substrate for the production of ABE, was studied in this article. First, physicochemical methods for detoxification such as nanofiltration and flocculation were applied and the best combination was ...

Protamine was investigated for its antibacterial activity against the periodontal pathogens, Porphyromonas gingivalis, Prevotella intermedia and Aggregatibacter actinomycetemcomitans. We determined the minimum inhibitory concentrations of protamine and its hydrolysate and their bactericidal activity. Protamine inhibited the growth of all periodontopathic bacteria tested on agar plates. Protamin...

hydrolysate, soy and conventional cow milk formulas on incidence of atopic disease in high risk infants. Annals of Allergy 1989; 63: 102–6. 4 Chandra R. Five-year follow-up of high risk infants with family history of allergy who were exclusively breastfed or fed partial whey hydrolysate, soy and conventional cow’s milk formulas. Journal of Pediatriatric Gastroenterology and Nutrition 1997; 24: ...

The aim of this study was to efficiently use barley straw as a lignocellulosic feedstock for biobutanol production. Dilute sulfuric acid pretreatment was employed to solubilize hemicellulose in barley straw from cellulosic residues. The pretreated hydrolysate was cofermented with starch-based biomass in acetone–butanol–ethanol (ABE) fermentation. There were two co-fermentation processes: I) Bar...