A micellar electrokinetic capillary chromatography (MECC) method has been developed for monitoring the myrosinase catalysed hydrolysis of 2-hydroxy substituted glucosinolates and the simultaneous formation of the corresponding degradation products (oxazolidine-2-thiones (OZTs) and nitriles). Glucosibarin ((2R)-2-hydroxy-2-phenylethylglucosinolate) was chosen as the model glucosinolate owing to ...

Pseudomonas fluorescens was grown on mineral salts media with phenol, p-hydroxybenzoic acid, p-hydroxy-phenylacetic acid, or p-hydroxy-trans-cinnamic acid as sole carbon and energy source. Each compound was first hydroxylated, ortho to the hydroxyl group on the benzene ring, to give catechol, protocatechuic acid (3,4-dihydroxy-benzoic acid), homoprotocatechuic acid (3,4-dihydroxy-phenylacetic a...

MATERIALS AND METHODS lndeno[1,2,3-cd]pyrene (IP) is a major environmental pollutant which is carcinogenic on mouse skin and in rat lung. Unlike benzo(a)pyrene, IP is a nonaltemant polycyclic aromatic hydro carbon which is devoid of a bay region. IP was mutagenic in Salmonella typhimurium TA100 in the presence of a 9000 x g supernatant from the livers of Aroclor-pretreated rats. Using a similar...

Lactate dehydrogenase and malate are the two main alpha-hydroxy acid dehydrogenases in human body. We investigated catalytic properties of lactate LDHC, LDHL6A MDH1 on aromatic α-keto acids phenylpyruvic acid, p-hydroxyphenylpyruvic 3,4-dihydroxyphenylpyruvic acid. The optimum temperatures for LDHL6A, 37 °C, 35 45 respectively; pH is 6.5, 5.5, respectively. Km LDHC were 0.90 mM 0.92 mM, has a h...

an efficient, simple and convenient route is described for the synthesis of biscoumarin (3,3'-(arylmethylene) bis (4-hydroxy-2h-chromen-2-one)) by using of recyclable catalyst tio2@ksf. in this method, we synthesis biscoumarin derivatives via 3multi-component reactions (3mcrs) of two equivalent 4-hydroxycoumarin with one equivalent of aromatic aldehydes using 20 mg nano tio2@ksf as homogen...

Aromatic polyketides from marine actinomycetes have received increasing attention due to their unusual structures and potent bioactivities. Compared to their terrestrial counterparts, marine aromatic polyketides have been less discovered and their structural and biological diversities are far from being fully investigated. In this study, we employed a PCR-based genome mining method to discover ...

Cleistanthol of the diterpenoid group is a newly isolated naturally-occuring molecule biological and pharmaceutical significance. The contians three ring structures, only one them aromatic, hydroxy- hydrocarbon- substitution groups modifying these rings. However, for this compound, synthetic method has not yet been proposed it similar structures sharing common structure but different sites cont...

The replacement of oxygenated functionality (hydroxy and alkoxy) with a fluorine atom is commonly used bioisosteric in medicinal chemistry. In this paper, we use molecular matched-pair analysis to better understand the effects on lipophilicity. It seems that reduced log P compound normally dominant determining size difference. We observe presence additional electron-donating groups an aromatic ...

the biodesulfurization (bds) of sulfur compounds in fossil fuels is a process to reduce sulfur dioxide emissions that cause environmental pollution. gordonia alkanivorans ripi90a is able to convert dibenzothiophene, an organic sulfur compound in petroleum, to 2-hydroxybiphenyl (2-hbp) in 4s pathway. in this study, (dbt), dsza and dszb, dbt sulfone monooxygenase, and desulfinase were respectivel...