The reaction of the hydroxyl radical, generated by a Fenton system, with pyrimidine deoxyribonucleotides was investigated by using the e.s.r. technique of spin trapping. The spin trap t-nitrosobutane was employed to trap secondary radicals formed by the reaction of the hydroxyl radical with these nucleotides. The results presented here show that hydroxyl-radical attack on thymidine, 2-deoxycyti...

The concentrations of ROS were measured in samples of the sensorimotor brain cortex and in the rat blood. We measured the following parameters: The six lines spectra, nitroxide radical, free hydroxyl radical and singleton oxygen. Their concentration was measured under physiological conditions, after the nociceptive stimulation and after the application of melatonin, both in normal and stimulate...

The methanolic extract of Sida retusa Linn.(Malvaceae),Urena lobata Linn.(Malvaceae)and Triumfetta rhomboidea Jacq.(Teliaceae) roots were found to inhibit lipid peroxidation, scavenge hydroxyl and superoxide radicals in vitro. The quantity of S.retusa root extract required for 50% inhibition of lipid peroxidation, scavenging hydroxyl radical and superoxide radical was 1130.24 ug/ml respectively...

Antioxidant activity of defatted methanol extract of D. malabarica bark was studied for its free radical scavenging property on different in vitro models e.g. 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide, superoxide, hydroxyl radical and lipid peroxide radical model. The extract showed good dose-dependent free radical scavenging property in all the models except in hydroxyl radical inhib...

The solubilization of glucose and several related sugars in benzene solutions of rigorously purified phosphatidylcholine has been studied; the equilibrium degree of solubilization has been found to depend on the size of the sugar molecule relative to that of the lecithin micelle, and on the number and the acidity of its free hydroxyl groups. The uptake of glucose increased as the lecithin conce...

Accumulated data have suggested that the hydroxyl groups of flavonoids are important for their bioactive function. To directly demonstrate the role of hydroxyl groups, we synthesized a derivative of quercetin, 3,7,3',4'-O-tetrabenzylquercetin (4Bn-Q) that substituted the hydroxyl groups of quercetin with benzyl groups, and then evaluated the ability to inhibit cell proliferation and cause apopt...

Present study was designed to compare antioxidant and DNA damage protection of Soxhlet assisted (80% methanol) extract (SEMC) with microwave assisted (80% methanol) extract of Michelia champaca Linn. (MEMC). The in vitro antioxidant activity of SEMC and MEMC were tested for DPPH, hydroxyl radical (OH) by deoxyribose, lipid peroxidation and reducing power capacity. The extracts were also tested ...

Liquid methanol at densities up to rhorho(0) = 1.7 was studied by NMR in a specially designed diamond-anvil cell. Methyl and hydroxyl resonances have been separately observed at pressures to 43 kbars which exceeds equilibrium freezing pressure of methanol. The chemical shift difference between methyl and hydroxyl protons increases nonlinearly with increasing density, indicating a noticeable dec...

Antibiotic mode-of-action classification is based upon drug-target interaction and whether the resultant inhibition of cellular function is lethal to bacteria. Here we show that the three major classes of bactericidal antibiotics, regardless of drug-target interaction, stimulate the production of highly deleterious hydroxyl radicals in Gram-negative and Gram-positive bacteria, which ultimately ...

Vertical and adiabatic excitation energies of the lowest (2)A(') excited state in the water-hydroxyl complex have been determined using coupled cluster, multireference configuration interaction, multireference perturbation theory, and density-functional methods. A significant redshift of about 0.4 eV in the vertical excitation energy of the complex compared to that of the hydroxyl radical monom...