The folding and oxidation of recombinant human granulocyte colony-stimulating factor solubilized from Escherichia coli inclusion bodies was investigated. During the folding process, two intermediates, I1 and I2, were detected by kinetic studies using high performance liquid chromatography. I1 exists transiently and disappears quickly with the concomitant formation of I2. In contrast, I2 require...

A phenomenon already discovered more than 25 years ago is the possibility of naïve helper T cells to polarize into TH1 or TH2 populations. In a simplified model, these polarizations occur at opposite ends of an "immune 1-2 axis" (i1-i2 axis) of possible conditions. Additional polarizations of helper/regulatory T cells were discovered later, such as for example TH17 and Treg phenotypes; although...

Esmolol has been shown to improve postoperative pain and reduce opioid requirements. The aim of this systematic review was to evaluate the effect of perioperative esmolol as an adjunct on early postoperative pain intensity, recovery profile, and anesthetic requirement. Databases were searched for randomized placebo-controlled trials evaluating the effects of esmolol during general anesthesia. P...

Abstract Background Patients having transcatheter aortic valve implantation (TAVI) routinely undergo coronary angiography before the procedure to define anatomy and evaluate extend of artery disease (CAD). Whether percutaneous intervention (PCI) prior/concomitant with TAVI confers any additional clinical benefit in patients CAD remains unclear. Purpose To compare outcomes PCI prior significant ...

We consider two simplicial arrangements of lines and ideals I intersection points these lines. There are 127 in both cases the numbers ti lying on exactly i configuration (points multiplicity i) coincide. show that one examples containment I(3)⊆I2 holds, whereas it fails other. also for a subarrangement 21 The interest relation between I(3) I2 ℙ2 is motivated by question posted Hochster Huneke ...

This study aims at investigating the acoustic correlates of supralaryngeal and muscular tension voice quality settings (VQS). Speech samples from 40 speakers were perceptually analyzed by means of the Vocal Profile Analysis Scheme (VPAS). Acoustic analysis comprised formant frequency (F1, F2 and F3) and intensity measurements (I1, I2 and I3) of seven Brazilian Portuguese vowels. Multivariate st...

Carriage of Helicobacter pylori strains producing more active (s1/i1) forms of VacA is strongly associated with gastric adenocarcinoma. To our knowledge, we are the first to determine effects of different polymorphic forms of VacA on inflammation and metaplasia in the mouse stomach. Bacteria producing the less active s2/i2 form of VacA colonized mice more efficiently than mutants null for VacA ...

The 14 November 2000, Ms8.1 Kokoxili earthquake is of huge influence in the world, which ruptured more than 400 km of the western end of the left-lateral Kunlun fault. The Kunlun fault is active and also after the heavy earthquake, it will definitely undergo an adjusting and balancing process. SAR Intereferometry (INSAR) is an effective measure for crustal deformation measurement even for minor...

Objective: evaluate the effectiveness of epinephrine used during cardiac arrest and its effect on the survival rates and neurological condition. Method: systematic review of scientific literature with meta-analysis, using a random effects model. The following databases were used to research clinical trials and observational studies: Medline, Embase and Cochrane, from 2005 to 2015. Results: ...