Concanavalin A-Sandwich ELISA (Con A-S-ELISA) was developed for the detection of infectious bronchitis virus (IBV) or chicken specific anti-viral antibodies. The antigen detection limit for the Con A-S-ELISA was 10(5,1) EID(50)/mL. Three homologous and four heterologous IBV strains were similarly detected. This assay was highly effective in detecting the virus after infected tissue homogenates ...

Infectious bronchitis virus (IBV) is distributed worldwide and causes significant losses in the poultry industry. In recent decades, lineages GI-19 GI-7 have become most prevalent IBV strains China. However, molecular evolution phylodynamics of lineage remain largely unknown. this study, we identified 19 from clinical samples January 2021 to June 2022 China, including 12 GI-19, 3 GI-7, 1 strain...

Infectious bronchitis virus (IBV) is an avian coronavirus affecting the respiratory tract of chickens. To analyze IBV infection of the lower respiratory tract, we applied a technique that uses precision-cut lung slices (PCLSs). This method allows infection of bronchial cells within their natural tissue composition under in vitro conditions. We demonstrate that IBV strains 4/91, Italy02, and QX ...

To understand the genetic diversity of the S2 gene of infectious bronchitis viruses (IBV) isolated in Japan, we determined the nucleotide sequences of these IBVs using the reverse transcriptase polymerase chain reaction method coupled with direct sequencing. IBV isolated in Japan were classified into six different groups by phylogenetic analysis based on the S2 gene. However, the classification...

Infectious bronchitis virus (IBV) is ubiquitous in most parts of the world where poultry are reared and is able to spread very rapidly in nonprotected birds. It is shed via both the respiratory tract and the faeces and can persist in the birds and faeces for several weeks or months. Although strict biosecurity and working with a one-age system are essential control measures, normally vaccinatio...

The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy r...

BACKGROUND Infectious Bronchitis is a highly contagious respiratory disease which causes tracheal lesions and also affects the reproductive tract and is responsible for large economic losses to the poultry industry every year. This is due to both mortality (either directly provoked by IBV itself or due to subsequent bacterial infection) and lost egg production. The virus is difficult to control...

Avian infectious bronchitis virus (IBV) causes high mortality and reduced production even in vaccinated birds. The study hypothesis was that a decoction of a Chinese herbal medicine, modified Ma Xing Shi Gan Tang, in the drinking water would reduce mortality and improve recovery and antioxidant capabilities in chickens infected with IBV. Two hundred 15-day-old chickens were randomly divided int...

to detect the presence of infectious bronchitis virus (ibv) in infected allantoic fluid (af) of spf embryonated eggs rapid hemagglutination (ha) activity after treatment with neuraminidase enzyme was used. twenty ibv suspected materials were inoculated in spf embryonated eggs via chorioallantoic cavity. harvested afs were treated with neuraminidase enzyme and the presence of ibv was detected by...

Introduction: Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is an acute and highly contagious disease in chickens. IBV is mainly considered a respiratory infection, other clinical manifestations, including renal, enteric and reproductive signs can be observed. Aim: Since there has been no study on evaluation of changes in biochemical parameters during IB infection, th...