Using isoelectric focusing (IEF) and radial immunodiffusion (RID) techniques, serum samples from 100 normal healthy adults and 21 patients with pulmonary emphysema were analysed to identify various alpha 1 antitrypsin phenotypes and the serum concentrations. Ten percent of the patients had low serum values. The normal or most common genetic form, MM, is the predominant phenotype in both control...

We developed a simple and highly-sensitive enzyme activity assay method based on reagent release capillary – isoelectric focusing (RRC-IEF), that can be applied for the development of multi analyte sensing device allowing simple and highly sensitive multi assay of both proteins and enzyme activities.

AIM To compare the costs and cost effectiveness of universal and targeted screening for the haemoglobinopathies; to compare the cost of two laboratory methods; and to estimate the cost effectiveness of programmes at different levels of prevalence and mix of haemoglobinopathy traits. METHODS A retrospective review of laboratory and follow up records to establish workload and costs, and estimat...

Electrophoretic methods can be used to identify meat of various animal species. The protein electrophoresis, especially the IEF of the sarcoplasmic proteins, is a wellestablished technique for species identification of raw fish and is used in the control of seafood authenticity. However, in the case of the analysis of heat-processed fish, the method is applicable only to those species which pos...

BACKGROUND α-1-Antitrypsin (A1AT) deficiency results from a genetic disorder at 2 common loci. Diagnosis requires quantification of A1AT and subsequent identification of the specific variant. The current algorithm of laboratory testing for the diagnosis of A1AT deficiency uses a combination of quantification (nephelometry), genotyping, and/or phenotyping. We developed a multiple reaction monito...

We investigate an axiomatization of the notion of common bel ief (knowledge) that makes use of no rules of inference (apart from Modus Ponens and Necessitat ion) and highl ight the property of the set of accessibi l i ty relat ions that characterizes each axiom. Mathernatics Subject Classif icat ion: 03845, 68T25.