Objective(s): The present work deals with the preparation of nanobioconjugates based on the immobilization of cytochrome c (cyt c) on functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect of the nanosupport and the immobilization procedure on the biochemical and structural characteristics of the immobilized protein was investigated. Methods: </strong...

A flow microdevice with programmable in situ formation/dissolution of a hydrogel 3D network was designed. An active porous medium shows excellent bio-compatibility and promises high, long-term activities without leaching immobilized biomolecules.

Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(ω-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme immobilisation and other biotechnological applications. Now, for the first time, we have found a fully reversible self-association (tetramerisation...

INTRODUCTION This study was conducted according to GCP criteria as a prospective randomized cross-over study. The primary goal of the study was to determine clinical findings and patient satisfaction with postoperative treatment. 29 patients with a distal radius fracture that was surgically stabilized from volar and who met the inclusion criteria were enrolled over a 12-month period. Each patie...

Transgenic zebrafish (Danio rerio) models of human diseases have recently emerged as innovative experimental systems in drug discovery and molecular pathology. None of the currently available technologies, however, allow for automated immobilization and treatment of large numbers of spatially encoded transgenic embryos during real-time developmental analysis. This work describes the proof-of-co...

BACKGROUND In order to match the challenges of quickly recognizing and treating any life-threatening injuries, the ABCDE principles were established for the assessment and treatment of trauma patients. The high priority of spine protection is emphasized by the fact that immobilization of the cervical spine is performed at the very first step in the ABCDE principles. Immobilization is typically ...

To the Editor:—We greatly appreciate and commend the recent study by Smith et al. comparing intubation difficulties in cervical spine immobilization with the use of the conventional laryngoscope and the WuScope (Achi Corp., Fremont, CA, and Asahi Optical Co.-Pentax, Tokyo, Japan). As the inventors of this relatively new device, we feel obligated to respond to issues raised by Smith et al. and t...

BACKGROUND Drop drying is a key factor in a wide range of technical applications, including spotted microarrays. The applied nL liquid volume provides specific reaction conditions for the immobilization of probe molecules to a chemically modified surface. RESULTS We investigated the influence of nL and μL liquid drop volumes on the process of probe immobilization and compare the results obtai...