Process hygiene plays a major role in the production of high quality beer. Knowledge of microorganisms found in the brewery environment and the control of microbial fouling are both essential in the prevention of microbial spoilage of beer. The present study examined the growth of surface-attached beer spoilage organisms and the detection and elimination of microbial biofilms. Moreover, the det...

Cockroaches carry microorganisms to sterilized materials, equipment and non-contaminated food in hospitals and in homes. Hence, they are regarded as major microbial vectors. We isolated and identified microorganisms from living cockroaches captured from a Brazilian health care institution and we determined the antimicrobial susceptibility profile of the isolates. Following collection, cockroach...

Some agricultural lands located in the Vromos Bay area, near the Black Sea coast, Southeastern Bulgaria, have been Ž . Ž . contaminated with radioactive elements uranium, radium and thorium and toxic heavy metals copper, cadmium and lead as a result of mining and mineral processing of polymetallic ores. Laboratory experiments carried out with soil samples from these lands revealed that an effic...

In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fluorescence lifetimes in DNA solutions than in RNA solutions. Growth experiment...

The main objective of this study is to evaluate qualitatively and quantitatively the effect of starvation periods in the biodegradation capacity of microorganisms when the support media is a material with high sorption capacity. Pollutant sorption and biodegradation, which occur simultaneously in the biofilter, describe the overall behavior of the air treatment system during normal operation an...

Accumulation of sorbitol in the tissue is known to cause microvascular diabetic complications. In this paper, a fiber-optic biosensor for sorbitol which is used as a biomarker of diabetic complications was developed and tested. The biosensor used a sorbitol dehydrogenase from microorganisms of the genus Flavimonas with high substrate specificity and detected the fluorescence of reduced nicotina...

Spent mushroom substrate (SMS) is the by-products of production, which mainly composed disintegrated lignocellulosic biomass, mycelia and some minerals. The huge output lack effective utilization methods make SMS becoming a serious environmental problem. In order to improve application derived biochar (SBC), composted (CSMS), SBC, combined plant growth-promoting rhizobacteria (PGPR, Bacillus su...

The advantages of immobilized over soluble enzymes arise from their enhanced stability and ease of separation from the reaction media, leading to significant savings in enzyme consumption. Immobilization methods range from binding to prefabricated carrier materials to packaging in enzyme crystals or powders. During their use, mass-transfer effects can produce substrate or pH gradients, which re...

Three-dimensional submicrometric structures and biomolecular patterns have been fabricated on a porous silicon film by an electron beam-based functionalization method. The immobilized proteins act as a passivation layer against material corrosion in aqueous solutions. The effects' dependence on the main parameters of the process (i.e., the electron beam dose, the biomolecule concentration, and ...

In the present study, Streptomyces gulbargensis and its mutant form, S.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of L-asparaginase using groundnut cake extract as medium. The medium with an initial pH of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oC and shaking at 200 rev/min....