Activation of C3 by nylon 6.6 and polymethyl methacrylate (PMMA) polymers in whole canine serum was investigated using Laurell electroimmunoassay and antigen-antibody crossed immunoelectrophoresis (XAA). Canine sera were utilized because most artificial blood contact materials used in prosthetic devices are tested in dogs. The measurements were made possible by converting the polymers to microp...

Crossed immunoelectrophoresis (CIE) and crossed immunoelectrofocusing (CIEF) were used to characterize proteins and mucosubstance in the saline extract of human ocular mucus pooled from the normal eyes of donors. CIE resolved more components with greater specificity than previous techniques. Up to 25 components were identified. Lactoferrin, protein G, tear prealbumin, and ocular mucosubstance w...

To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The results show that anti-M. leprae antibodies were synthesized in vitro in the bio...

Wright, D. N. (Iowa State University, Ames), and W. R. Lockhart. Environmental control of cell composition in Escherichia coli. J. Bacteriol. 89:1026-1031. 1965.-Escherichia coli was grown in continuous culture at various rates in a defined medium with either the carbon or the nitrogen source as rate-limiting substrate. Average cell size and the cellular content of ribonucleic acid, protein, an...

Now that something is beginning to be known about the functions of the individual serum proteins there is need of a simple and satisfactory way of estimating their concentrations in normal and pathological conditions. It is difficult to do this by physico-chemical methods since serum is a mixture of so many different components. Methods such as electrophoresis distinguish about five main groups...