BACKGROUND Even though porphyria cutanea tarda is the most frequent type of porphyria, there are few studies about its cutaneous physiopathology. OBJECTIVE To evaluate skin changes in porphyria cutanea tarda using light microscopy and direct immunofluorescence before and after treatment with chloroquine. To perform antigen immunomapping of bullae to study their level of cleavage. METHODS Li...

serologic diagnostic of hydatid cyst by immuno-fluorescence technique of the serological methods for the diognosis of hydatid cystimmuno-fl uoresccnt technique using fro zen section of the sco lex as antigen has given encouraging results. in t he present study examina t io n o f 8 5 sera correspond ing to h yd atido se patien ts by t his met hod, has shown th at 94% of th e cas es can be d e te...

A new recombinant antibody, RB611, detects human and murine pancreatic polypeptide (PPY) by immunofluorescence on histological sections.

The recombinant antibodies AS739, AT693 and AU734 detect by immunofluorescence the spike S protein from SARS-CoV-2.

Control of the genetic information encoded by DNA in mammalian chromosomes is mediated by proteins, some of which are only transiently attached, although others are intrinsically associated with nucleic acid in the complex mixture known as chromatin. Chromatin-associated proteins range from the ubiquitous and abundant histones down to the most specific and rare of transcription factors. Althoug...

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respect...

A positive serological diagnosis of amoebiasis could be made by immunofluorescence in 66 of 78 established cases, taking a serum titre of 16 or higher as diagnostic: at this level there were no false positives among 94 control sera. The test is simple and may be carried out on amoebic smears stored for several months in 2-octanol. The serological activity is largely confined to the IgG immunogl...

We have studied the localization of Australia antigen, a particulate substance associated with hepatitis, by means of the fluorescent antibody technique. Preparations were made from 61 liver biopsy specimens taken from patients with infectious hepatitis, serum hepatitis, and a variety of other diseases. When tested with fluorescein-conjugated rabbit anti-Au(1) antisera all 26 patients who had A...