The development of an immunosensor-based biosensor to detect the human serum albumin (HSA) was developed as a low-cost and label-free electrical detection through silicon nitride ion-sensitive field-effect transistor (Si3N4-ISFET). This sensor functionalized using 3-Aminopropyltriethoxysilane (APTES). silanized Si3N4-ISFET surface covalently linked with antibody against HSA (Anti-HSA) via gluta...

A proof of concept for the simultaneous multi-parameter determination of three inflammation and sepsis parameters-TNFα, PCT and CRP-using a compact optical immunosensor is demonstrated. Harmonized assay conditions revealed standard curves with test midpoints (IC(50)) of 380 µg L(-1) for TNFα, 2300 µg L(-1) for PCT, and 2645 µg L(-1) for CRP.

The first electrochemical immunosensor (EI) for the detection of antibodies against deamidated gliadin peptides (DGP) is described here. A disposable nanohybrid screen-printed carbon electrode modified with DGP was employed as the transducer's sensing surface. Real serum samples were successfully assayed and the results were corroborated with an ELISA kit. The presented EI is a promising analyt...

A multi-functional luminol-reduced Pt@Au hybrid flower-like nanocomposite (luminol-Pt@AuNF) which not only acts as an efficient signal probe but also constitutes a pseudobienzyme amplifying system with choline oxidase (ChOx) was firstly synthesized and applied to the construction of a solid-state luminol electrochemiluminescence (ECL) immunosensor for cardiac troponin I (cTnI) detection.

In this work, we proposed a novel electrochemical immunosensor for sensitive detection of the total protein of Nosema bombycis based on Fe3O4 nanoparticles (Fe3O4NPs) as catalyst to electrocatalyze the reduction of methylene blue (MB) with the aid of Fe3O4NPs-DNA dendrimers for the signal amplification.

An immunosensor based on the electrochemiluminescence (ECL) of luminol was proposed by coupling enzymatic reaction to in situ generate coreactant with Pd nanoparticles as catalyst for the ECL reaction, which was successfully applied for the ultrasensitive detection of α-1-fetoprotein with a low detection limit of 33 fg mL(-1).

Acute kidney injury is among the most severe health problems today, with greatest fatality ratios. The molecule-1 (KIM-1) considered to be a potential biomarker for diagnosis of acute injury. Herein, sensitive, selective, and swift sandwich-type electrochemical KIM-1 immunosensor was fabricated based on porous NiCo2S4@CeO2 microspheres as signal amplifier covalent organic frameworks-gold nanopa...

A novel electrochemical immunosensor for the detection of important marine biotoxins domoic acid (DA) and okadaic (OA) was developed. The sensors used carbon black modified screen-printed electrodes (CB-SPE) obtained using a high-throughput method. performance stability CB SPEs bare (c-SPEs) were compared cyclic voltammetry impedance spectroscopy. CB-SPEs showed improved long-term (at least six...

The highly enhanced electrochemiluminescence from a novel hybrid gold/silica/CdSe-CdS quantum-dot nanostructure has been reported for the first time, and successfully applied to develop an ultrasensitive ECL immunosensor for the detection of a protein tumor marker, carcinoembryonic antigen.