background: despite widespread clinical uses of resin-modifi ed glass-ionomers (rmgis), their sealing ability is still a concern. this study evaluated the effect of delayed light activation (dla) of rmgi on marginal sealing in differently pretreated cavities. materials and methods: in this in vitro study, two standardized class v cavities were prepared on the buccal and lingual surfaces of 56 s...

Introduction: Cutaneous Leishmaniasis (CL) is a parasitic disease caused by Leishmania species. Currently accessible treatments remain insufficient, and there is pressure to develop suitable and effectual options. In this study, we used different concentrations of podophyllin in vitro on leishmania parasites and then on leishmaniasis lesions in mice and compared their efficacy. Method: We used ...

Background : Propylene glycol (1,2-propanediol, PG) is a commonly used solvent for oral, intravenous, as well as topical pharmaceutical preparations. While PG is generally considered to be safe, it has been known that large intravenous doses given over a short period of time can be toxic. Objective: To evaluate the effect of PG in sepsis induced by the bacterial endotoxin lipopolysaccharide (LP...

T cells triggered to blast transformation and proliferation by histoincompatible cells have the capacity of reverting "back" to lymphocytes. These "secondary" lymphocytes and their progeny cells are able to respond repeatedly to the same allogeneic stimulus in vitro.

Immediate-type drug hypersensitivity is an increasingly significant clinical issue; however, the diagnosis is frequently hindered due to lack of safe and precise diagnostic tests. Flow cytometry-assisted basophil activation test is a safe in vitro diagnostic tool for assessing basophil activation upon allergen stimulation. In this review, we have summarized current literature on the diagnostic ...

Sera from dogs that were hyporesponsive to interferon (IFN) induction blocked virus induction of IFN in spleen cells in vitro but not IFN action in dog kidney cells. Similarly, macrophages or their culture fluids were found to block IFN induction in lymphoid cells in vitro. Lymphoid cells deprived of phagocytic cells secreted IFN continuously for 4 days, whereas lymphoid cells in the presence o...

PlXDOSO: TO assess the possible role Of experimental uveitogenic antigens (S-Ag, IRPB and recoverin) in human uveitis. Methods : Patients suffering from non-infectious bilateral UVeitiS and from the Ocular type of Behcet's disease were included in this study. Patients’ lymphocytes were tested for their possible in vitro anamnestic immune ~~SpClllS~ (specific blast transformation) to S-Ag, IRBP ...

The in vitro blastogenesis response of lymphocytes from 19 patients, with either visceral or mucocutaneous leishmaniasis or Chagas disease, to antigens of the Leishmania-Trypanosoma complex was studied. Cells from all patients responded to both homologous and heterologous antigens and the magnitude of the responses did not differ for any of the patient groups or antigens.

The present studies have been carried out to characterize the regulatory influences acting upon defined pathways of T cell-dependent B cell activation. In these studies, it was demonstrated that high concentrations of free carrier strongly inhibited the MHC-restricted in vitro T cell-dependent antibody responses of primed Lyb-5- B cells to the corresponding carrier-hapten conjugate. In contrast...

Intravenously applied human IgG has beneficial effects in treating inflammatory diseases, presumably because it has a complement attenuating role. This role of IgG was studied in vitro by following C3 activation and inactivation in sera that were supplemented with exogenous human IgG and incubated with immune aggregates. IgG added at 2 to 10 mg/mL stimulated the physiologic inactivation of C3b-...