Background: Levan or fructan, a polysaccharide of fructose, is widely used in various commercial industries. Levan could be produced by many organisms, including plants and bacteria. The cloning of the gene from Bacillus licheniformis, which expressed levansucrase in E. coli host, was carried out successfully.  In the present study, we performed the in vitro production of levan and analyzed its...

Background: Mentha piperita L. essential oils have different antibacterial activity. In the present study, we investigated the effect of &gamma–irradiation on the antibacterial activities of Mentha piperita L. essential oils in vitro and in vivo systems. Materials and Methods: The aerial parts of peppermint were irradiated in a cobalt60 source with 0, 10 and 25 kGy absorbed doses. Then, t...

callus and suspension cultures of lavandula angustifolia mill. (lamiaceae) were established and the effect of different culture media on growth rate was investigated. terpenoids added to suspension culture to investigate their biotrans-formation. all samples were analyzed by gas chromatography (gc) and gc-mass spectroscopy (ms). octan-1-ol, citronellol, linalool, borneol and geraniol were biotr...

background cutaneous leishmaniasis (cl) is an endemic disease in developing countries. although pentamidine orantimonite (glucantime) has been recommended for cutaneous leishmaniasis treatment by the world health organization, there are some concerns too such as high cost, side effects, need for frequent injections, and restricted efficacy. therefore, different methods have been used for cl tre...

nanotechnology is a rapidly growing field having potential applications in many areas. nanoparticles (nps) have been studied for cell toxicity, immunotoxicity, and genotoxicity. tetrazolium-based assays such as mtt, mts, and wst-1 are used to determine cell viability. cell inflammatory response induced by nps is checked by measuring inflammatory biomarkers, such as il-8, il-6, and tumor necrosi...

candidiasis , the main opportunistic fungal infection has been increased over the past decades. this study aimed to characterize c.albicans species complex ( c.albicans , c.dubliniensis , and c.africana ) isolated from patients with respiratory infections by molecular tools and in vitro antifungal susceptibilities by using broth microdilution method according to clsi m27-a3 guidelines. totally,...