In the present study, the in vitro response of MM.106 apple rootstock to increasing concentrations of NaCl (0, 20, 40, 80, 100 and 120 mM) in the Murashige and Skoog culture medium was analyzed. Explant growth was seriously affected by salinity treatments. Elevated salinity from 20 (control) to 40, 80, 100 and 120 mM NaCl resulted in reduction in shoot growth (shoot number, length and fresh wei...

A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of Canavalia gladiata (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L – 1.5mg/...

To enhance the genetic resource base of Echinacea purpurea (L.) Moench, a tetraploid was developed by treating seeds with different concentrations of colchicine. For further multiplication, in vitro micropropagation through adventitious shoot induction and proliferation was achieved. Shoot buds were induced directly on adaxial surface of mature leaf tissues growing on MS medium supplemented wit...

considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

The effect of plant growth regulators on shoot proliferation from shoot tip explants of Ajuga multiflora was studied. The highest number of shoots (17.1) was observed when shoot tip explants were cultured on Murashige and Skoog (MS) medium fortified with 8.0 µM 6-Benzyladenine (BA) and 2.7 µM α-naphthaleneacetic acid (NAA). The mean number of shoots per explant was increased 1.6-fold in liquid ...

The conventional methods of propagation of bamboos sexual as well as vegetative have lots of problems that restrict their multiplication in large numbers. Bamboo is now a commercial crop and hence there is shortage of availability of planting stock material. To overcome this tissue culture offers rapid and reliable method to produce plants in large number. Tissue culture protocol has been devel...