We have investigated the effects of hyperosmolarity induced by sucrose on the fluid phase endocytosis of the fluorescent dye lucifer yellow CH (LY) and the endocytosis of 125I-asialo-orosomucoid (ASOR) by the galactosyl receptor system in isolated rat hepatocytes. Continuous uptake of LY by cells at 37 degrees C is biphasic, occurs for 3-4 h, and then plateaus. Permeabilized cells or crude memb...

The sink-source conversion in developing leaves of tobacco (Nicotiana tabacum L.) was studied to determine whether import termination is caused by the onset of export or is related to achievement of positive carbon balance. Albino shoots were grown in vitro and grafted to detopped stems of green tobacco plants. Termination of import was studied by providing mature leaves of the stock plant with...

Spinach (Spinacia oleracea L.) leaf discs accumulated free proline when exposed to polyethylene glycol solutions of water potential less than -10 bars. At -20 bars, the accumulation was 11 micromoles per gram original fresh weight in a 24-hour period.When the leaf organelles were separated on a sucrose gradient, a proline oxidase was detected in the mitochondrial fraction. Isolated mitochondria...

achillea millefolium belonging to the astreaceae family is an endangered medicinal plant of jordan and of its neighboring countries. as an alternative to seed propagation, an efficient micropropagation of a. millefolium and its subsequent rooting were developed as an option for its in vitro conservation. a maximum of 5.9 shoots per microshoot were obtained on murashige and skoog agar medium sup...

Surfactant protein (SP) A and SP-A-mediated lipid uptake by isolated type II cells were investigated with biochemical and morphological methods. Inhibition of coated-pit function by potassium depletion severely reduced both SP-A and SP-A-mediated lipid internalization. Lipid uptake in the absence of SP-A was not affected. With confocal laser scanning microscopy and immunoelectron microscopy, SP...

iran is one of the main date (phoenix dactylifera) producing countries in the world. however, little information is available on the pollen viability and favorable storage conditions of iranian male date cultivars. this study was conducted to estimate the best in vitro pollen germination of three male date palm cultivars, `ghanami’, `samsmavi’ and `gheibani’ using various doses of boric acid (0...

1. The effect of overloading of hamster fibroblast lysosomes with sucrose on the turnover of lysosomal and Golgi-apparatus enzymes was studied. Arylsulphatase B and UDPgalactose-N-acetylglucosamine galactosyltransferase were chosen as appropriate marker enzymes. The relative contributions ofchanges in the rates of synthesis and degradation to the increased activities ofthese enzymes after uptak...

Background: Since the freezing method was offered, much effort has been done to improve the freezing conditions in different studies and the impact of different freezing methods and mediums on oocyte, embryo and ovarian tissues has been examined. Based on studies, minerals play an important role in oocyte maturation and subsequent embryo development. Vanadium is one of the minerals, which is ve...

(22S,23S)-Homobrassinolide was tested for its effect on the electric cell potential, proton extrusion, ferricyanide reduction, and amino acid and sucrose uptake of leaves of Egeria densa Planchon. In the light, (22S,23S)-homobrassinolide and its derivative, 2alpha-3alpha-dihydroxy-5alpha-stigmast-22-en-6-one, were similar to each other and similar to fusicoccin in causing hyperpolarization and ...