We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA. An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants can supplement base pairing to specify miRNA target recognition. In a four-genome analysis of 3' UTR...

Some rodents produce ultrasonic vocalizations (USVs) for social communication using an aerodynamic whistle, a unique vocal production mechanism not found in other animals. The functional anatomy and evolution of this sound production mechanism remains unclear. Using laryngeal airway reconstruction, we identified anatomical specializations critical for USV production. A robust laryngeal cartilag...

Comprehensive next-generation sequencing (NGS) panels for cancer diagnostics create a bottleneck interpretation. QIAGEN Clinical Insights Interpret One (QCI) is clinical decision support software that supports molecular pathologists in the classification of oncology-related variants. This study compares variant assessments by QCI to utilizing current laboratory methods. Eight laboratories were ...