Analysis of fructose and glucose from glucose isomerization process using immobilised glucose isomerase (IGI), {Sweetzyme from Novozymes} are often performed by HPLC methods with refractive index (RI) detector. This study is focused on developing new methods of measuring glucose and fructose using a specific carbohydrate column. The importance of this research, primarily based on the performanc...

For the evaluation of autoantibodies in Pemphigus vulgaris (PV), an indirect ELISA assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (M.W.) of 130-160 kDa. The evaluation of Pemphigus IgG, IgM and IgA autoantibodies in 75 patients and 50 control sera indicated that this indirect ELISA assay was a useful method for the detection of ...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...

Recent American Food and Drug Administration guidelines have effectively determined that mixtures of chiral compounds can no longer be brought to the pharmaceuticals marketplace. These guidelines require a means for chiral detection and compound separation. This article describes separation and detection of chiral pharmaceuticals using HPLC with circular dichroism detection. Two over-the-counte...

We carry out a comparison between different kinds of experiments for the detection of neutralino dark matter. In particular, we compare the theoretical predictions for the direct detection of these particles, through their elastic scattering on target nuclei through nuclear recoils, with those for their indirect detection through the gamma rays produced by their annihilation in the galactic cen...

We report on the conception, design, fabrication, experimental characterization, and theoretical analysis of nanocrystal scintillators hybridized on Si detectors and CCDs for enhanced detection and imaging in UV. Integrating luminescent CdSe/ZnS core-shell nanocrystals (NCs of 3.2 and 5.2 nm in diameter with 5% size distribution) to efficiently convert incident UV radiation to visible emission,...