Persistent infection with Japanese encephalitis virus (JEV) was established in murine neuroblastoma N18 cells, and the persistency has been maintained in cell culture for over 6 months. From the persistently infected cells, a clone named C2-2 was selected and expanded to form a stable cell line. The vast majority of C2-2 cells showed viral protein staining by immunofluorescence and continuously...

Two nasopharyngeal carcinoma (NPC) cell lines and one keratinocyte cell line could be infected with Epstein-Barr virus (EBV) by cocultivation with virus-producing cells but not by cell-free virus. Using porous culture inserts to manipulate the cell-to-cell contact, we demonstrated that contact between EBV donor B cells and EBV recipient epithelial cells was required for the infection. Cell-to-c...

Enzyme immunoassay (EIA) systems for measles virus and rubella virus were studied from the standpoints of requirements for suitable viral antigens and control antigens, and the sensitivity and specificity of the tests for detecting antibody elicited by past infection (determination of immunity status), and for serodiagnosis of currenet infections. Crude or semipurified measles virus antigens we...

In studies of the susceptibility of various primary tissue culture systems to arthropod-borne virus infection, it was found that host cell reactions induced by certain members of this group of viruses may take a variety of forms under the influence of various conditions.8'9""' Thus, a single virus strain may stimulate a different type of cellular response in each of two equally susceptible cult...

mycoplasma and ureaplasma species are the most serious contaminants of cell cultures and this remains one of the major problem encountered in biological research using cell cultures. the aim of this study was to investigate the rate of mycoplasma and a total of 100 different cell culture specimens including, r228, hela, vero, mdck, l20, rd and hep2 were collected. then the specimens were invest...

The aim of this study was to evaluate the immunomodulatory property sauerkraut (in presence and absence Lactobacillus casei culture) on Escherichia coli infected Balb -C mice. Fermentation freshly washed, shredded cabbage fulfilled by adding 2% salt prepare ‘sauerkraut control’, whereas ‘inoculated sauerkraut’ prepared salt, sugar 20% culture. After incubation for five days at room temperature,...

A glycoprotein of an apparent molecular mass of 46,000, gp 46, was enriched by affinity chromatography from the virus- and cell-free culture medium of adult T-cell leukemia virus (ATLV) infected cells. gp 46 was specifically precipitated with sera from patients with adult T-cell leukemia associated antigen (ATLA). Thus, gp 46 is a novel component of the ATLA antigen complex.

Co-culture of prion-infected cells with uninfected target cells has recently been found to lead to rapid and efficient transmission of infectivity to the target cells by a process that is dependent on direct cell contact.

A human osteogenic sarcoma cell culture is useful in the immunofluorescence serological test for detecting human cytomegalovirus antibody. These sarcoma cells are chronically infected with human cytomegalovirus and provide a constant number of immunofluorescence-positive cells.

The development of Isospora belli, a human coccidian parasite, was studied in different cell lines. Merozoites were observed in all kinds of cells, whereas sporogony was demonstrated only in Hct-8. This implied that not only the human cell line can be infected, but also some animal cell lines. Unizoites could be found in Vero cells. The merozoites were transferred to a new culture cell for thre...