Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies against seasonal H1N1 and H3N2 influenza were measured in children and adolescents (n = 656) by ...

This study aims to assess the comparative cytotoxic activity of ethanolic extract Mikania cordata (MC), micrantha (MM) and scandens (MS) (Family: Asteraceae) using brine shrimp lethality bioassay colorimetric sulforhodamine B assay method. In SRB assay, A549 human lung carcinoma, SK-Mel-2 skin melanoma B16F1 mouse cell lines were used. LC50 for MC, MM, MS vincristine found be 29.04, 15.84, 32.3...

This study aimed to synthesize chitosan/polyvinyl alcohol (CS/PVA)-based zinc oxide (ZnO) and titanium dioxide (TiO2) hybrid bionanocomposites (BNCs) observe their comparative accomplishment against the skin cancer cell line, A431, antioxidant potential. CS was blended with PVA form polymeric films reinforced immobilization of ZnO TiO2 nanoparticles (NPs), separately. The optimization BNCs done...

A radioinhibitor binding assay and an enzyme inhibition assay have been developed to measure plasma levels of CGP 38 560, a potent human renin inhibitor. The detection limit of the assays was between 0.5 and 1 pmol/ml. There was a good correlation (r = 0.989) between the two assays for the measurement of human plasma spiked with CGP 38 560 in concentrations from 1.9 nM to 12 microM. Intra-assay...

We investigated the antimutagenic activities and chemical compositionsof essential oil (EO) from Melissa officinalis, Lavandula angustifolia Origanum onites plants belonging to Lamiaceae family. The compositions of were determined by GC/MS analysis Ames assay was used in activity analysis. Citral, linalool, thymol terpinenes major components EOs M. L. O. onites. In assay, it that EO officinalis...

Bacterial resistance to antibiotics makes previously manageable infections again disabling and lethal, highlighting the need for new antibacterial strategies. In this regard, inhibition of bacterial division process by targeting key protein FtsZ has been recognized as an attractive approach discovering antibiotics. Binding small molecules cleft between N-terminal guanosine triphosphate (GTP)-bi...

Phytochemical analysis of the ethanolic extract leaves Cucurbita maxima indicated presence reducing sugars, alkaloids, saponins, tannin, steroid and glycosides whereas Euphorbia royleana glycosides, steroids tannin. The possible analgesic anti-oxidant activities were investigated. exhibited inhibition writhing reflex 16.13% 41.94% respectively (P<0.001 P<0.001) 19.35% 45.16% (P<0.02 on...

testosterone level was determined in six different populations of iran using saliva steroid assay. significant difference was observed in morning and afternoon testosterone level of the same individual (p>0.00l) significant difference was also noticed among different regions studied (p>0.00 1). comparisons between alcoholics and nonalcoholic as well as smokers and nonsmokers showed significant ...

In vitro, the differentiation of megakaryocytes (MKs) is improved by aryl-hydrocarbon receptor (AHR) antagonists such as StemRegenin 1 (SR1), an effect physiologically recapitulated presence stromal mesenchymal cells (MSC). This inhibition promotes amplification a CD34+CD41low population able to mature MKs with high capacity for platelet production. this short report, we showed that emergence t...

In this work, twelve analogues of piperidine alkaloids (-)-cassine and (-)-spectaline were synthesized, as well the racemic forms these natural products. The compounds evaluated for their inhibition electric eel acetylcholinesterase (AChEee) human butyrylcholinesterase (BChEhu) by on-flow mass-spectrometry-based dual-enzyme assay, mechanisms most potent also determined. Our results showed a pre...