Paldimycin (U-70138F) is a new antimicrobial agent with activity against gram-positive cocci. Clinical isolates of staphylococci and streptococci were tested. MICs were higher in Mueller-Hinton broth than in nutrient broth. Change in pH had minimal effect on the MICs in either broth. When inoculum size was varied, an inoculum effect was observed. The gram-positive cocci tested were generally mo...

Specific alloantibody admixed with a grafted murine lymphoma is suppressive of the graft in mice of the inbred strain native to the tumor. Suppressive capacity of the host is obviated in mice given 500 R whole body irradiation before tumor inoculation but is restored when normal peritoneal macrophages are admixed with the tumor-antibody inoculum. Other normal cell types admixed with the tumor-a...

The human T- and B-cell lines HPBMa10-2 and Daudi produced infectious hepatitis C virus (HCV) for more than 1 year after infection. The infectivity titer of the cell culture-grown HCV and its genome titer were comparable. The virion density in sucrose was around 1.12 g/ml. Among the 13 variants detected in the inoculum, 7 were adsorbed by the cells and one particular HCV sequence which was pres...

Tests with 10-micrograms amdinocillin disks were performed in parallel with agar dilution tests and three different ranges of inoculum density. A resistance breakpoint of less than or equal to 13 mm and an intermediate category of 14 to 16 mm are recommended. Susceptible strains provided zones greater than or equal to 17 mm in diameter, but many appeared to be resistant by the agar dilution met...

We compared the nodulation competitiveness of three strains of Rhizobium leguminosarum by counting the number of nodules formed on faba bean plants after the application at sowing time of different concentrations of the strains to soils already containing Rhizobium strains of the same species. A relationship of type y = ax was found to exist between the ratio of the nodules formed by the applie...

In this study, factors such as pH, temperature, carbon and nitrogen sources that affect mycelial growth of Hydnum repandum, a medicinal mushroom, were investigated. Different inoculum media for vegetative inoculum production were also examined. The best suitable pH for mycelial growth was found to be 5.5. Among constant temperatures, the best mycelial growth was obtained at 20 and 25°C. The myc...

Intersite variation in ectomycorrhizal (ECM) inoculum potential in soils from 16 sites located in arid subalpine areas of the White Mountains of California was quantified. The study sites included valleys dominated by big sagebrush (Artemisia tridentata Nutt.) and mountainsides dominated by ancient Great Basin bristlecone pine (Pinus longaeva Bailey). ECM inoculum potential was not detected at ...

A technique for inoculation with precise numbers of fungal spores on leaves and stems of plants was developed. The technique consisted of placing 1-μl drops with a fixed number of spores on the surface of leaves and stems, and covering each inoculum drop with a 10-μl drop of low-temperature gelling SeaPlaque agarose to fix the inoculum on the target site. With this technique single zoospores of...

Minimum inhibitory concentrations of ceftizoxime for seven clinical isolates of Bacteroides fragilis were found to be markedly affected by inoculum size; the very low minimum inhibitory concentration values (less than or equal to 0.25 microgram/ml) obtained in tests with most strains were not sustained when the inoculum was raised 100-fold. Benzylpenicillin and cephalothin were also affected by...