As in most invertebrate microvillar photoreceptors, phototransduction in Drosophila melanogaster uses a G-protein-coupled phosphoinositide pathway, whereby hydrolysis of phosphatidyl inositol 4,5-bisphosphate (PIP(2)) by phospholipase C generates inositol 1,4,5-trisphosphate (InsP(3)) and diacyl glycerol (DAG), leading to activation of two classes of Ca(2+)-permeable light-sensitive channel, en...

BACKGROUND Guidelines recommend mechanical ventilation with Intermitted Positive Pressure Ventilation (IPPV) during resuscitation. The influence of the novel ventilator mode Chest Compression Synchronized Ventilation (CCSV) on gas exchange and arterial blood pressure compared with IPPV was investigated in a pig model. METHODS In 12 pigs (general anaesthesia/intubation) ventricular fibrillatio...

The potential roles of protein tyrosine kinases (TKs) and of phosphotyrosine phosphatases (PTPs) in pancreatic islet function are not known. In this study, we investigated whether vanadate, a potent PTP inhibitor, affects phosphoinositide (PI) metabolism by a TK-dependent pathway in isolated mouse islets. To avoid the confounding effects of changes in Ca2+ influx, all experiments were performed...

Odor stimulation may excite or inhibit olfactory receptor neurons (ORNs). It is well established that the excitatory response involves a cAMP transduction mechanism that activates a non-selective cationic cyclic nucleotide-gated (CNG) conductance, accompanied by the activation of a Ca 2+-dependent Cl-conductance, both causing a depolarizing receptor potential. In contrast, odor inhibition is du...

Higher plants share with animals a responsiveness to the Ca(2+) mobilizing agents inositol 1,4,5-trisphosphate (InsP(3)) and cyclic ADP-ribose (cADPR). In this study, by using a vesicular (45)Ca(2+) flux assay, we demonstrate that microsomal vesicles from red beet and cauliflower also respond to nicotinic acid adenine dinucleotide phosphate (NAADP), a Ca(2+)-releasing molecule recently describe...

The inclusion of insoluble NSP (iNSP) in weaner pig diets has been reported to decrease post-weaning colibacillosis (PWC). Conversely, soluble NSP (sNSP) have been shown to exacerbate PWC. The present study investigated the effect of NSP solubility and inclusion level on the health and performance of newly weaned pigs challenged with enterotoxigenic Escherichia coli (ETEC), using NSP sources kn...

______________________________________ a Center for Evaluation Research & Surveys, Division of Health Economics National Institute of Public Health (INSP) / Mexican School of Public Health Av. Universidad 655, Cuernavaca, Mexico, CP 62508 b Institute of Business and Economic Research (IBER) c School of Public Health, Health Policy & Management d Haas School of Business University of California,...

A conceptual system to produce 3D thermal models of tires for tire inspection and defect characterization is proposed. The system uses registered range and thermal information to build highly detailed 3D models using either a volumetric or meshbased approach. To achieve this goal, two narrow bandpass filters are used in conjunction with two IR cameras to obtain the true temperature of the targe...

Calcium-binding protein 1 (CaBP1) regulates inositol 1,4,5-trisphosphate receptors (InsP(3)Rs) and a variety of voltage-gated Ca(2+) channels in the brain. We report complete NMR chemical shift assignments of the Ca(2+)-saturated form of CaBP1 with Ca(2+) bound at EF1, EF3 and EF4 (residues 1-167, BMRB no. 16862).

The inositol 1,4,5-trisphosphate (InsP(3)) receptor (InsP3R) is an endoplasmic reticulum-localized Ca2+ -release channel that controls complex cytoplasmic Ca(2+) signaling in many cell types. At least three InsP3Rs encoded by different genes have been identified in mammalian cells, with different primary sequences, subcellular locations, variable ratios of expression, and heteromultimer formati...