Video recordings of receptionists at work in general practice were found to be useful for self assessment by the receptionists and enabled the doctors to see areas for improvement in the organization of the reception area.

Lower input resistance with intracellular recording, rather than with whole cell recording, usually has been ascribed to a shunt produced by penetration injury. An alternative explanation is a higher input resistance during whole cell recording due to wash-out of cytoplasmatic substances. We have used neuronal polarization at the onset and termination of an applied electric field for shunt dete...

OBJECTIVE To elucidate the role of the membrane potential in human detrusor smooth muscle contraction, by simultaneously recording mechanical and intracellular electrical activity in muscle strips. Materials and methods The agonists acetylcholine and carbachol were applied to induce a contraction on muscarinic receptor stimulation; to block the response, atropine was added to the bath. The Ca2+...

Recording intracellular (IC) bioelectrical signals is central to understanding the fundamental behaviour of cells and cell networks in, for example, neural and cardiac systems. The standard tool for IC recording, the patch-clamp micropipette is applied widely, yet remains limited in terms of reducing the tip size, the ability to reuse the pipette and ion exchange with the cytoplasm. Recent effo...

The freshwater leech, Hirudo medicinalis, is a versatile model organism that has been used to address scientific questions in the fields of neurophysiology, neuroethology, and developmental biology. The goal of this report is to consolidate experimental techniques from the leech system into a single article that will be of use to physiologists with expertise in other nervous system preparations...

Synaptic efficacy is modified following a brief train of high frequency stimulation (HFS) to a cell's afferent fibers (long-term potentiation, LTP). Modulation of synaptic efficacy could be achieved through changes in excitability of interneurons. To test this hypothesis, we used the in vitro hippocampal slice to record intracellularly from CA1 basket cell interneurons before and following a pe...

Intracellular responses in anterior middle suprasylvian gyrus to peripheral stimuli (light flash, auditory click, and contralateral forepaw shock) and central stimuli (contralateral cortical and tha!amic stimulation) were studied in cats anesthetized with chloralose. When cells discharged to peripheral and some central stimuli, spikes arose from asynchronous, summating, depolarizing postsynapti...

Developing an In Vivo Intracellular Neuronal Recording System for Freely Behaving Small Animals by Inho Yoon Department of Electrical and Computer Engineering Duke University Date:_______________________ Approved: ___________________________ Bruce R. Donald, Supervisor ___________________________ Richard Mooney ___________________________ Gleb Finkelstein ___________________________ James Moriz...

With intracellular recording techniques, we investigated evoked responses and electrophysiological properties of neurons in anterior cingulate gyrus (ACG) to stimulating greater splanchnic nerve (GSN) in cats. Among 461 neurons, 176 were stimulus-relative and 285 stimulus-irrelative. According to the characteristics of the evoked responses, stimulus-relative neurons were classified into specifi...

In the present study, we investigated the auditory responses of the medial geniculate (MGB) neurons, through in vivo intracellular recordings of anesthetized guinea pigs, while the auditory cortex was electrically activated. Of the 63 neurons that received corticofugal modulation of the membrane potential, 30 received potentiation and 33 received hyperpolarization. The corticofugal potentiation...