Fig. 1. Electropherogram stained for alkaline phosphatase isoenzymes A. albumin with added biiWubin; B, billrubin solution In saline; C, patient’s serum with artifactual band in addition to placental and lIver isoenzyme bands (poody resolved); D. intestinal and placental isoenzyme control; E, patient’s serum with liver Isoenzyme band; F, Intestinal and live’ lsoenzyme control; 0, patient’s seru...

Lactate dehydrogenase C, an isoenzyme composed of C polypeptide subunits and found only in mature testes and spermatozoa, differs kinetically, chemically and immunologically from the five common isoenzymes of lactate dehydrogenase, each of which is a tetramer of A and/or B subunits. In the rat lactate dehydrogenase C exists in two molecular forms, isoenzymes C4 and A1C3. In addition to these tw...

An epidemic of sleeping sickness, which started in 1976 in a focus within the county of Luuka in Central Busoga, has spread to cover the three districts of Busoga and large parts of the neighbouring districts of Tororo and Mukono. Forty-three isolates of the subgenus Trypanozoon from Busoga and Tororo (27 from man, 9 from cows, 2 from pigs and 5 from tsetse flies) were compared by thin-layer st...

Background. The technogenic pollution leads to excessive production of reactive oxygen species (ROS) in plants which are highly and toxic cause damage biomolecules. Plants have a complex antioxidant defense system that protects cells from the ROS maintain homeostasis. most important link this is enzymes, particular, peroxidase. It was interest determine expression protective properties one sens...

A flat-bed apparatus for preparative gel electrophoresis with intermittent elution [l-31 permits the collection simultaneously of anodeand cathode-migrating components of a mixnue. We employed this first for the separation of isoenzymes of lactate dehydrogenase (EC 1.1.1.27) in crude tissue extracts. Enzyme activity was determined by reaction rate observed at 340 nm in the presence of 0.14 mM N...

The molecular structure of human foetal intestinal alkaline phosphatase was defined by high-resolution two-dimensional polyacrylamide-gel electrophoresis and amino acid inhibition studies. Comparison was made with the adult form of intestinal alkaline phosphatase, as well as with alkaline phosphatases isolated from cultured foetal amnion cells (FL) and a human tumour cell line (KB). Two non-ide...

In this assay for lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 (LD-1), 1,6-hexanediol is added to serum after total LD has been measured. After incubation for 5 min the total LD remaining is determined. Samples from patients who had a myocardial infarction or who had undergone bypass surgery were assayed simultaneously by the 1,6-hexanediol method, the Roche "Isomune LD," and by electrophore...

We have studied five patients who have exhibited an unusual alkaline phosphatase isoenzyme (ALP EC 3.1.3.1) migrating in an ultrafast position electrophoretically on cellulose acetate. This ALP isoenzyme has been identified in patients with benign and malignant liver diseases. In addition, a number of these patients exhibited a regular ALP liver isoenzyme and a fast (preliver) ALP isoenzyme in ...

Evidence of acute pancreatitis was sought in 35 patients with fulminant hepatic failure. Total amylase was raised in 22 patients and isoenzyme separation showed a distinct P3 isoenzyme (indicative of pancreatitis) in 14. In four patients with marked hyperamylasaemia (greater than 1000 U/l) the predominant isoenzyme was the salivary fraction. Pancreatic lipase was abnormally raised (greater than...

Evidence is presented in this paper which supports the hepatogenic theory for the mechanism by which the level of serum alkaline phosphatase is raised in liver disease and provides additional evidence that serum phosphatase is not excreted in the bile. By starch gel and paper electrophoresis the normal serum alkaline phosphatase isoenzyme is shown to be rarely present in hepatic bile. The actio...