The role of the liver in the disposition of circulating mercapturic acids was examined in anesthetized rats and in the isolated perfused rat liver using S-2,4-dinitrophenyl- N-acetylcysteine (DNP-NAC) as the model compound. When DNP-NAC was infused into the jugular vein (150 or 600 nmol over 60 min) it was rapidly and nearly quantitatively excreted as DNP-NAC into bile (42-36% of the dose) and ...

The isolated perfused rat kidney experiment was introduced in 1959 for studying the regulation of renal blood flow and is recognized as a valuable preparation for studying physiological and biochemical aspects of renal function such as hemodynamics, glomerular filtration rate (GFR) and overall handling of fluids. Dose-response curves are obtained by injection of bolus doses of drugs into the pe...

Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1 -3 hr) incubation periods. The hepatocytes release very low density (d < 1.01 g/ml) lipoprotein (VLDL) particles, which as a group a) contain triacylglycerols, phospholipids, free cholesterol, and cholesteryl esters in molar proportions of 100:21:8:4; b) have a mean diameter 1.5-fold larger than...

BACKGROUND Discordant xenotransplantation, the grafting of organs from one phylogenic species to another, results in hyper-acute rejection (HAR). HAR is associated with the deposition of recipient preformed xenoreactive natural antibodies and complement on the endothelium of the donor organ, leading to activation and apoptosis of the endothelium, an event associated with xenograft rejection. En...

The possible functional significance of induction of the hepatic enzymes, tryptophan pyrrolase and tyrosine transaminase, has been studied in intact normal and adrenalectomized adult male rats and in the isolated perfused rat liver. In intact, normal rats, several-fold increases in tryptophan pyrrolase activity induced by treatment with hydrocortisone or tryptophan, or both, were associated wit...

The possible functional significance of induction of the hepatic enzymes, tryptophan pyrrolase and tyrosine transaminase, has been studied in intact normal and adrenalectomized adult male rats and in the isolated perfused rat liver. In intact, normal rats, several-fold increases in tryptophan pyrrolase activity induced by treatment with hydrocortisone or tryptophan, or both, were associated wit...

The overall purpose of this study was to investigate Neurostan Saint John’s Wort (SJW) on the disposition of fexofinadine in the isolated perfused rat liver. SpragueDawley (SD) rats (n = 16) randomized into 3 groups, including: control, low dose (Hypericum, one type of neutostan) and high dose group. Each animal among these groups was pre-treated with either vehicle (ethanol in Milli Q water at...