Loop 52-72 of porcine fructose-1,6-bisphosphatase may play a central role in the mechanism of catalysis and allosteric inhibition by AMP. The loop pivots between different conformational states about a hinge located at residues 50 and 51. The insertion of proline separately at positions 50 and 51 reduces k(cat) by up to 3-fold, with no effect on the K(m) for fructose 1,6-bisphosphate. The K(a) ...

Although K-ras is frequently mutated in lung adenocarcinomas, the normal function of K-ras p21 in lung is not known. In two mouse (E10 and C10) and one human (HPL1D) immortalized lung cell lines from peripheral epithelium, we have measured total K-ras p21 and active K-ras p21-GTP during cell proliferation and at growth arrest caused by confluence. In all three cell types, total K-ras p21 increa...

Glutamate dehydrogenase (NAD) activity was measured in liver and diaphragm mitochondria from 48 h fasted rats. Kinetic studies were performed with diaphragm enzyme and the effects of L-leu, ADP and L-ala on the K(1)m and V(1)max for NH(4)+, and α-ketoglutarate were evaluated. L-leucine increases by 2-8 fold the V(1)max for all three substrates with no significant changes in the K(1)m. ADP incre...

Decomposable mappings from the space of symmetric k-fold tensors over E, ⊗ s,k E, to the space of k-fold tensors over F , ⊗ s,k F , are those linear operators which map nonzero decomposable elements to nonzero decomposable elements. We prove that any decomposable mapping is induced by an injective linear operator between the spaces on which the tensors are defined. Moreover, if the decomposable...

Maintenance and repair of the bridge are inevitable in operation a to maintain its condition keep operation. Indonesia has hundreds thousands bridges that still actively use. The classic problem with infrastructure management, such as bridges, is large numbers generally not balanced adequate maintenance budgets. Therefore,the strategy implementing by preparing priorities becomes only logical ap...

When using machine-learning techniques to determine algorithms or ranking models that identify student satisfaction, are often trained and tested on a single data set, leading bias in their performance metrics. This article aims the best algorithm classify satisfaction of university students applying K-fold cross-validation technique, comparing error rates metrics before after its application. ...

Our objective was to alter the substrate specificity of purine nucleoside phosphorylase such that it would catalyse the phosphorolysis of 6-aminopurine nucleosides. We modified both Asn-243 and Lys-244 in order to promote the acceptance of the C6-amino group of adenosine. The Asn-243-Asp substitution resulted in an 8-fold increase in K(m) for inosine from 58 to 484 microM and a 1000-fold decrea...

Luminescence Nanothermometry Selecting green fluorescent protein and Ag2S nanocrystals as illustrative multiparametric thermographic phosphors, in article number 2100301, Luís D. Carlos co-workers demonstrate that the synergy between readouts multiple linear regression makes possible a 10-fold improvement performance of luminescent thermometers reaching world record relative thermal sensitivity...