We have recently reported the identification of kringle 1-5 (K1-5) of plasminogen as a potent and specific inhibitor of angiogenesis and tumor growth. Here, we show that K1-5 bound to endothelial cell surface ATP synthase and triggered caspase-mediated endothelial cell apoptosis. Induction of endothelial apoptosis involved sequential activation of caspases-8, -9, and -3. Administration of neutr...

Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to three different human cancers: Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. The Kaposi's sarcoma lesion expresses high levels of angiogenic factors and is comprised of a mixed cell population, including endothelial cells that are infected with KSHV. We find that the KSHV K1 protein is expressed in ...

Amemiya, Morimasa, Kaoru Tabei, Eiji Kusano, Yasushi Asano, and Robert J. Alpern. Incubation of OKP cells in low-K1 media increases NHE3 activity after early decrease in intracellular pH. Am. J. Physiol. 276 (Cell Physiol. 45): C711–C716, 1999.—Chronic hypokalemia increases the activity of proximal tubule apical membrane Na1/H1 antiporter NHE3. The present study examined the effect of the incub...

Neonatal meningitis, caused by Escherichia coli K1, is a serious central nervous system disease. We have established that macrophages serve as permissive niches for E. coli K1 to multiply in the host and for attaining a threshold level of bacterial load, which is a prerequisite for the onset of the disease. Here, we demonstrate experimentally that three N-glycans in FcγRIa interact with OmpA of...

BACKGROUND Primary pyogenic liver abscess (PLA) caused by Klebsiella pneumoniae is an emerging infectious disease. Capsular serotype K1 and the magA gene have been reported to be associated with this disease. METHODS The prevalence of magA was determined by polymerase chain reaction (PCR). The sequences of the magA flanking region were completed by inverse PCR and direct sequencing. Serotypin...

We isolated PCR, RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE-PCR)-, and RT-PCR-generated clones from mouse kininogen family transcripts. DNA sequencing indicated that the clones were from two distinct genes. One set (K1) is from the previously reported mouse kininogen gene. The second set (K2) has an open reading frame, is 93% identical to K1 in the overlapping nucleotide seq...

To investigate the physiological role of a kidney-specific chloride channel (ClC-K1), we sought to determine its exact localization by immunohistochemistry and its functional regulation using Xenopus oocyte expression system. The antiserum specifically recognized a 70-kD protein in SDS-PAGE of membrane protein from rat inner medulla and an in vitro translated ClC-K1 protein. Immunohistochemistr...

1. In the neurogenic heart of the isopod crustacean Porcellio dilatatus, external K+ removal depolarized the membrane (K0 effect) whereas subsequent restoration of K+ resulted in a rapid hyperpolarization (K1 effect). 2. The amplitude of the K1 effect depended on the duration of the prior K+ deprivation and on the subsequent K+ concentration. 3. The membrane resistance slightly increased during...

In this paper, we rigorously prove the existence and stability of K-peaked asymmetric patterns for the Gierer-Meinhardt system in a two dimensional domain which are far from spatial homogeneity. We show that given any positive integers k1, k2 ≥ 1 with k1 + k2 = K, there are asymmetric patterns with k1 large peaks and k2 small peaks. Most of these asymmetric patterns are shown to be unstable. Ho...

For s ≥ 3 a graph is K1,s-free if it does not contain an induced subgraph isomorphic to K1,s. Cycles in K1,3-free graphs, called clawfree graphs, have beenwell studied. In this paper we extend results on disjoint cycles in claw-free graphs satisfying certain minimum degree conditions to K1,s-free graphs, normally called generalized claw-free graphs. In particular, we prove that if G is K1,s-fre...