www.cmj.hr Aim To investigate whether ATP-sensitive potassium (KATP) channels modulate the tocolytic effect of β2-adrenergic receptor (β2-AR) agonists (ritodrine and salmeterol) in early-pregnant (day 6) and late-pregnant (day 22) rat uterus in vitro, in order to examine the relation between the KATP channel sulphonylurea-binding regulatory subunit (SUR) expression and pharmacological reactivit...

Both mitochondrial ATP-sensitive K+(KATP) channels and the actin cytoskeleton have been proposed to be end-effectors in ischemic preconditioning (PC). For evaluation of the participation of these proposed end effectors, rabbits underwent 30 min of regional ischemia and 3 h of reperfusion. PC by 5-min ischemia + 10-min reperfusion reduced infarct size by 60%. Diazoxide, a mitochondrial KATP-chan...

The KATP channels play an important role in the membrane excitability and vascular tone regulation. Previous studies indicate that the function of KATP channels is disrupted in oxidative stress seen in a variety of cardiovascular diseases, while the underlying mechanism remains unclear. Here, we demonstrate S-glutathionylation to be a modulation mechanism underlying the oxidant-mediated vascula...

background: renal ischemia reperfusion (ir) injury has been a major source of concern during the past decades and angiotensin converting enzyme (ace) inhibitors have been successfully used to prevent this injury. there have been some controversial reports about the involvement of katp channels in the mechanism of action of ace inhibitors. in this study, we examined the effect of katp channel bl...

INTRODUCTION Levosimendan is approved for left heart failure and is also used in right heart failure to reduce right ventricular afterload. Despite the fact that pulmonary arteries (PAs) and pulmonary veins (PVs) contribute to cardiac load, their responses to levosimendan are largely unknown. MATERIALS AND METHODS Levosimendan-induced vasorelaxation of PAs and PVs was studied in precision-cut...

Transcripts of a gene, GIRK4, that encodes for a 419-amino-acid protein and shows high structural similarity to other subfamily members of G-protein-activated inwardly rectifying K+ channels (GIRK) have been identified in the human hippocampus. When expressed in Xenopus oocytes, GIRK4 yielded functional GIRK channels with activity that was enhanced by the stimulation of coexpressed serotonin 1A...

ATP-sensitive potassium (KATP) channels have been suggested to contribute to coronary and skeletal muscle vasodilation during exercise, either alone or interacting in a parallel or redundant process with nitric oxide (NO), prostaglandins (PGs), and adenosine. We tested the hypothesis that KATP channels, alone or in combination with NO and PGs, regulate exercise hyperemia in forearm muscle. Eigh...

Chan, Y.-L., Orie, N. N., Singer, M., and Clapp, L. H. (2007). Inflammatory mediators enhance expression of vascular ATP-sensitive potassium channels. Intensive Care Med. 33:S120. Chan, Y.-L., Orie, N. N., Singer, M., and Clapp, L. H. (2008). Enhanced expression of vascular KATP channels in an in vitro model of sepsis. Fundam. Clin. Pharmacol. 22:S34. Chan, Y.-L., Stidwill, R., Taylor, V., Clap...

BACKGROUND Volatile anesthetics produce differing degrees of myocardial protection in animal models of ischemia. The purpose of the current investigation was to determine the influence of isoflurane and halothane on myocardial protection in a human model of simulated ischemia and the role of adenosine A1 receptors and adenosine triphosphate-sensitive potassium (KATP) channels in the anesthetic ...

Storey NM, Stratton RC, Rainbow RD, Standen NB, Lodwick D. Kir6.2 limits Ca overload and mitochondrial oscillations of ventricular myocytes in response to metabolic stress. Am J Physiol Heart Circ Physiol 305: H1508–H1518, 2013. First published September 6, 2013; doi:10.1152/ajpheart.00540.2013.—ATP-sensitive K (KATP) channels are abundant membrane proteins in cardiac myocytes that are directly...