The concentration of intracellular free calcium [Ca2+]i in human platelets was measured by the quin-2 method. 5-Hydroxytryptamine (5-HT) at 10(-5) M induced a rapid transient increase of [Ca2+]i which was antagonized by 10(-7) M ketanserin or cyproheptadine. The verapamil derivative, desmethoxyverapamil (D888), showed stereospecific inhibition of the 5-HT-induced [Ca2+]i increase. The IC50 for ...

Biochemicals 5-hydroxytrptophan, 5-hydroxy indole acetic acid, ketanserin, bicuculline, baclofen, atropine, sodium octyl sulfonic acid, ethylene diamine tetra acetic acidEDTA, D-glucose, calcium chloride, bovine serum albumin fraction V, Superoxide dismutase from bovine erythrocytes were purchased from Sigma Chemical Co., St. Louis, USA. All other reagents were of analytical grade purchased loc...

The effect of endogenous serotonin on long-term potentiating (LTP) in region CA1 was studied by blocking 5-HT2 receptors with ketanserin in rat hippocampal slices. Such a block significantly enhanced long-term potentiation of the CA1 population spike induced by high frequency stimulation of the schaffer collateral/ commissural pathway. This implies that serotonin acts on 5-HT2 receptors in CA1 ...

Amaç: Bu çalışmada, farelerde beta-sitosterol uygulamasına bağlı santral analjezide opioiderjik, serotonerjik, adrenerjik ve kolinerjik reseptörleri ile ilişkili olası antinosiseptif aktivitesi araştırılmıştır. Gereç Yöntem: Beta-sitosterol, farelere 5, 10 20 mg/kg dozlarında uygulandı. Daha sonra, fareler beta-sitosterolün etkilerini araştırmak için tail-flick hot-plate testleri analiz edildi....

Moringa stenopetala (Baker f.) Cufod., is an endemic species growing in the south of Ethiopia. M. often consumed as food and used traditional medicine it has also been traditionally for relieving pain This study aimed to investigate antinociceptive effect mechanisms action leaves methanol extract mice. The per-oral doses 50, 100, 200 mg/kg were tested by using hot-plate, tail-immersion, writhin...