For the precise assay of minute quantities of insulin in biological fluids, techniques using fibril formation (1) or paper chromatography (2), although specific for the insulin molecule, lack the required sensitivity. Methods based on the measurement in vivo of a fall in blood sugar in surgically modified rats (3) afford sensitivity, but lack both specificity and precision. In zvitro biological...

This investigation has sought to determine the significance of the wide range of extractable collagen fractions which appear to exist in growing connective tissues and to determine their position in the process of fibrogenesis. Carrageenin granulomata were induced in guinea pigs and, after injection of (14)C-glycine, this tissue and skin from the same animal were subjected to successive extract...

Ashwagandha (W. somnifera) is widely known as the queen of Indian Ayurveda. The objective this study was to determine antioxidant and antibacterial activity different parts Ashwagandha. extracts from determined using DPPH free radical scavenging method. Ashwaghanda root inhabiting bacteria isolated pour-plate technique. agar well method filter paper were followed assess extracts. Ethanol-water ...

A simplified method is presented for the determination of butanol-extractable iodine (BEI). The results compare favorably with the original method as described by Man et al. Also available, as part of the procedure for BEI, is a direct chemical determination for butanol-insoluble iodine (Bli) from the same specimen of serum. Heretofore, nonbutanol-extractable iodine has been studied only by mea...