INTRODUCTION 363 KI.NETOPLAST MINICIRCLE DNA 365 Is There a Gene Product? 365 Sequence Organization in Different Species 365 Sequence Heterogeneity as a Diagnostic and Epidemiological Tool 368 Analysis of Conformational Bend in Minicircle DNA 370 Replication of Minicircle DNA 371 KINETOPLAST .MAXICIRCLE DNA 372 Mitochondrial Ribosomal R.VA genes 372 Mitochondrial Siruciural Genes and Open Readi...

Trypanosoma brucei's mitochondrial genome, kinetoplast DNA (kDNA), is a giant network of catenated DNA rings. The network consists of a few thousand 1 kb minicircles and several dozen 23 kb maxicircles. Here we report that TbPIF5, one of T. brucei's six mitochondrial proteins related to Saccharomyces cerevisiae mitochondrial DNA helicase ScPIF1, is involved in minicircle lagging strand synthesi...

Lindera megaphylla has been traditionally used as an antineoplastic and wound healing remedy. We previously demonstrated the antitumor effects of D-dicentrine, a natural aporphine alkaloid from the root of L. megaphylla. To generate analogues, series of phenanthrene alkaloids from D-dicentrine were synthesized by degradation with ethyl chloroformate in pyridine, base hydrolysis, and N-alkylatio...

The fine structure of epimastigotes of Trypanosoma cruzi and promastigotes of Herpetomonas megaseliae was analysed in replicas of quick-frozen, freeze-fractured, deeply etched and rotary-replicated cells. Using control cells and cells treated with Triton X-100 before glutaraldehyde fixation, images were obtained that showed connections of the sub-pellicular microtubules with each other, with th...

Diaryl-furanones are specific analytical reagents for the biochemical detection of primary amines by fluorescence techniques. Well-known fluorescamine (Fluram) and 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF), yielding fluorescent products with λem at 480–490 nm. Although reaction claim to be pyrrolinones, recent studies show that they really 3-oxopyrrole (pyrrolone) derivatives. Both have been...

The phylogenetic relationships of avian trypanosomes, common parasites of birds, remain ambiguous and validity of many species is questionable. Analyses based on 18S rRNA sequences, dimensions of the kinetoplast disc and the size of kinetoplast (k) DNA minicircles were used to differentiate among large trypanosomes parasitizing birds of the Old World. These trypanosomes with typical striated ap...

In this study, we report the characterization of seven trypanosome stocks isolated in different geographical areas of Latin America and from different vector species, by analysis of kinetoplast DNA (kDNA) restriction fragmentlength polymorphism, using five different restriction endonucleases, and genomic DNA by duplex PCR assay. According to the statistical study, the stocks were grouped into t...

Molecular topology of polymers plays a key role in determining their physical properties. We studied herein the topological effects on static and dynamic properties 2D catenated network DNA rings called kinetoplast. Restriction enzymes that cleave at sequence-specific sites are used to selectively cut remove from hence tune molecular while maintaining overall structural integrity. find has mini...

objective: molecular epidemiology of visceral leishmaniasis (vl) is currently used widely for different objectives such as vector incrimination studies. materials and methods: in this study three different loci including kinetoplast dna (kdna), ribosomal dna (rdna), and cystein protease b (cpb) of leishmania parasite genome were used for detection and identification of natural infection of san...