Mu bacteriophage inserts its DNA into the genome of host bacteria and is used as a model for DNA transposition events in other systems. The eukaryotic Ku protein has key roles in DNA repair and in certain transposition events. Here we show that the Gam protein of phage Mu is conserved in bacteria, has sequence homology with both subunits of Ku, and has the potential to adopt a similar architect...

Our main goal in this chapter is to show that the spectrum KU representing periodic complex K-theory has an H~ structure. The existence of such a structure is important since it will allow us to develop a complete theory of Dyer-Lashof operations in K-theory, including the computation of K,(O3(); this program is carried out in chapter IX. Of course, we already know that the connective spectrum ...

Werner syndrome (WS) is a premature aging disorder where the affected individuals appear much older than their chronological age. The single gene that is defective in WS encodes a protein (WRN) that has ATPase, helicase and 3'-->5' exonuclease activities. Our laboratory has recently uncovered a physical and functional interaction between WRN and the Ku heterodimer complex that functions in doub...

Repair of single-ended DNA double-strand breaks (seDSBs) by homologous recombination (HR) requires the generation of a 3' single-strand DNA overhang by exonuclease activities in a process called DNA resection. However, it is anticipated that the highly abundant DNA end-binding protein Ku sequesters seDSBs and shields them from exonuclease activities. Despite pioneering works in yeast, it is unc...

Single-ended double-strand breaks (DSBs) are a common form of spontaneous DNA break, generated when the replisome encounters a discontinuity in the DNA template. Given their prevalence, understanding the mechanisms governing the fate(s) of single-ended DSBs is important. We describe the influence of the Ku heterodimer and Mre11 nuclease activity on processing of single-ended DSBs. Separation-of...

1,2 Hiroyuki Kanazawa, * Naoki Onishi, * Yuri Mizusawa, * Takahiro Tsunekawa *6,*7 Hitohide Usami * author 1 Technical Computing Solution Unit, Fujitsu Limited, Nakase 1-9-3, Mihama-ku, Chiba-city, Chiba 261-8588, Japan, [email protected] 2 Graduate School of Natural Science & Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan *3 Technical Computing Solut...

The magnetocrystalline anisotropy Ku of continuous Co1−xPtx alloy films peaks at 30 at. % of Pt. Further increase in Pt at. % decreases Ku quickly probably due to the increasing density of stacking faults SFs and the fcc phases. The effect of substrate bias has been studied in various compositions of CoPt films. Ku increases from 8 10 6 to 1.1 107 ergs /cm3 when 50 V bias was applied to Co60Pt4...

Aberrant activation of Akt plays a pivotal role in cancer development. ATM, a protein deficient in patients with ataxia-telangiectasia disease, is traditionally considered as a nuclear protein kinase that functions as a signal transducer in response to DNA damage. It has recently been shown that ATM is also a cytoplasmic protein that mediates the full activation of Akt in response to insulin. O...

KU-32 is a novel, novobiocin-based Hsp90 inhibitor that protects against neuronal glucotoxicity and reverses multiple clinical indices of diabetic peripheral neuropathy in a rodent model. However, any drug with potential for treating diabetic complications must also have no adverse effects on the function of pancreatic islets. Thus, the goal of the current study was to assess the effect of KU-3...

Terminal deoxynucleotidyl transferase (TdT) catalyzes the addition of nucleotides at the junctions of rearranging Ig and T cell receptor gene segments, thereby generating antigen receptor diversity. Ku is a heterodimeric protein composed of 70- and 86-kDa subunits that binds DNA ends and is required for V(D)J recombination and DNA double-strand break (DSB) repair. We provide evidence for a dire...