Elaboration of an optimized kinetic test for urinary alanine aminopeptidase at 37 degrees C is described. As final concentrations, 50 mmol/L tris(hydroxymethyl)aminomethane x HCl buffer, pH 7.8, and 2 mmol/L alanine-4-nitroanilide are proposed. Five- to sixfold activitiy is obtained as compared with a generally used test procedure. Coefficients of variation for the method (within-run and betwee...

Horse erythrocytes are polymorphic with respect to L-alanine permeability. The present investigation compared the specificity, kinetics and cation-dependence of erythrocyte amino acid transport in two groups of thoroughbred horses, those with erythrocyte L-alanine permeabilities in the range 5-15 mumol/h per litre of cells (0.2 mM extracellular L-alanine, 37 degrees C) (transport-negative type)...

Measurements of alanine aminotransferase and aspartate aminotransferase with the SMAC were evaluated for correlation with the ABA-100, precision, linearity, and carryover. We assayed 200 specimens with normal and abnormal aminotransferase activities with both the SMAC (y) and the ABA-100 (X). Linear regression analysis of the data yielded the following: alanine aminotransferase (r = 0.9732, y =...

Alanine and glutamine uptake by the liver of 50-52-day-old genetically obese Zucker rats and their lean littermates has been studied. The net uptake in vivo of L-alanine is 2-fold higher in the obese animals. No significant change in L-glutamine net balance was found. We also studied the Na(+)-dependent uptake of L-alanine and L-glutamine into plasma-membrane vesicles isolated from either obese...

Keynan, A. (University of Wisconsin, Madison), W. G. Murrell, and H. O. Halvorson. Germination properties of spores with low dipicolinic acid content. J. Bacteriol. 83:395-399. 1962.-When the dipicolinic acid content of spores of Bacillus cereus strain T is reduced from 7.5 to 2 or 3%, the spores germinate spontaneously after heat activation and are sluggish in their response to l-alanine and o...

UNLABELLED Vancomycin resistance in Gram-positive bacteria results from the replacement of the D-alanyl-D-alanine target of peptidoglycan precursors with D-alanyl-D-lactate or D-alanyl-D-serine (D-Ala-D-Ser), to which vancomycin has low binding affinity. VanT is one of the proteins required for the production of D-Ala-D-Ser-terminating precursors by converting L-Ser to D-Ser. VanT is composed o...

1. Aminoacylase is irreversibly inactivated by the chloromethylketone analogs of benzyloxy-carobonyl-L-alanine, L-alanine, L-leucine, L-aspartic acid (beta), tosyl-L-phenylalanine and L-leucyl-L-alanine. The kinetics of the inactivation of the enzyme by the halo-methylketones were investigated. 2. Leucyl-and alanyl chloromethylketone inactivate the enzyme by blocking of 4 SH groups. Experiments...

As a follow-up to our effort establish reliable thermodynamic data for amino acids, the heat capacity and phase behavior are reported N-acetyl glycine amide (CAS RN: 2620-63-5), N-acetyl-L-alanine 15962-47-7), N-acetyl-L-valine 37933-88-3), N-acetyl-L-isoleucine 56711-06-9), N-acetyl-L-leucine 28529-34-2). Prior measurement, thermogravimetric analysis X-ray powder diffraction were performed det...